氧化海藻酸钠交联壳聚糖载药复合体系的制备及其药物缓释初步研究

Preparation of oxidized alginate sodium cross-linked chitosan scaffold for drug delivery

为获得一种新型的药物释放复合体系,本实验首先通过乳化交联法制备壳聚糖(CS)包载四环素(TC)微球,然后利用氧化海藻酸钠交联聚磷酸钙/壳聚糖(CPP/CS)复合材料,用冷冻干燥法制备了载药微球复合体系。并用傅立叶红外光谱仪(IR)、扫描电镜(SEM)及药物的体外释放等方法对该载药微球复合体系进行分析和表征。结果显示,经过氧化海藻酸钠交联的CPP/CS复合材料中无机相均匀分散在连续有机相基体中,制备的CPP/CS空白复合体系与载药微球复合体系具有较为理想的孔隙结构和贯通性。其中的微球均呈球状,粒径分布在5～50μm之间,微球表面光滑且比较致密,载药后有利于减缓药物的突释效应。载药微球复合后,微球在复合体系中分布均匀,而且与CPP/CS材料之间亲合性较好,其药物的缓释效果得到明显的提高。实验将为制备出具有能满足药物缓释材料的需要,又能进行骨缺损的修复的双功能的复合药物载体进行了初步探索。

To obtain a novel composite drug delivery system（DDS）,chitosan（CS） microspheres were firstly prepared by emulsion cross-linking method.Then these microspheres mixed with calcium polyphosphate（CPP）/CS composite particles were crosslinked with oxidized alginate sodium（ADA） by using the method of freeze-drying.The properties were characterized by the methods of infrared spectra（IR）,scanning electronic microscope（SEM） and in vitro drug release.The results shows that CPP are distributed uniformly into CS phase and properties of CPP and CS are stable in the DDS.Pores are distributed uniformly in the system and have high interconnection.CS microspheres（5-50μm） in the system have smooth surface and dense spherical integrity.This research will offer a new kind of carrier with good properties for bone tissue engineering and for the sustained release of drug which will have great potential to be used for bone or cartilage repair.