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RP-HPLC法测定吲哚美辛在人工结肠液中的含量 被引量:3

Content Determination of Indomethacin in Artificial Colon Juice by RP-HPLC
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摘要 目的:建立测定自制吲哚美辛结肠靶向胶囊在人工结肠液中的吲哚美辛含量的方法。方法:采用反相高效液相色谱法。色谱柱为Hypersil ODS2 C18柱,流动相为乙腈-0.1mo·lL-1冰醋酸(60∶40),流速为1mL·min-1,柱温为25℃,检测波长为228nm,进样量为20μL。结果:吲哚美辛检测浓度在2.0~50.0μg·mL-1范围内与峰面积积分值线性关系良好(r=0.9999);低、中、高浓度加样回收率分别为100.37%、99.87%、100.00%(n=3),RSD分别为0.29%、0.31%、0.74%。结论:本文所建立的吲哚美辛在人工结肠液中的含量测定方法准确、可靠。 OBJECTIVE: To establish the method for the content determining of Indomethacin colon-targeting capsule in artificial colon juice. METHODS: RP-HPLC method was adopted. The determination was performed on Hypersil ODS2 C18 column with mobile phase consisted of acetonitrile-0.1 mol·L-1 glacial acetic acid(60 ∶ 40) at flow rate of 1 mL·min-1. The detection wavelength was set at 228 nm and the column temperature was 25 ℃. The injection volume was 20 μL. RESULTS:The linear range of indomethacin was 2.0~50.0 μg·mL-1(r=0.999 9). The recovery rates of it at low, medium and high concentrations were 100.37%,99.87%,100.00% with RSD of 0.29%, 0.31%, 0.74%, respectively. CONCLUTION: The method is accurate, reliable and precision for the content determination of indomethacin in artificial colon juice.
出处 《中国药房》 CAS CSCD 北大核心 2011年第20期1901-1903,共3页 China Pharmacy
基金 温州市科技计划项目(S20100042)
关键词 吲哚美辛 反相高效液相色谱法 人工结肠液 含量测定 Indomethacin RP-HPLC Artificial colon juice Content determination
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