脓毒症大鼠有核细胞过氧化物酶体增殖物激活受体γ活性与白细胞介素-6的关系

The relationship of peroxisome proliferator activated receptor γ ligands activity in nucleated cell and interleukin-6 level in septic rats

目的观察脓毒症时有核细胞过氧化物酶体增殖物激活受体γ（PPART）活性、血浆促炎介质白细胞介素-6（IL-6）的变化，探讨两者的关系。方法按随机数字表法将90只SD大鼠分为正常对照组、假手术组、脓毒症组，每组再分为术后12、24、48h3个亚组，每组10只。采用盲肠结扎穿孔术（CLP）制作大鼠脓毒症模型，用酶联免疫吸附法（ELISA）测定有核细胞PPAR7活性及血浆IL-6水平。结果脓毒症组术后12、24、48h有核细胞PPARγ活性（4值：0．279±0．004、0．264±0．009、0．245±0．012）较正常对照组（0．292±0．007、0．293±0．004、0．293±0．005）和假手术组（0．295±0．008、0．295±0．006、0.294±0．007）明显下降，而血浆IL-6水平（ng／L：365．25±15．53、507．16±20．86、437．89±25．09）较正常对照组（43．54±11．10、48．82±10．62、42．96±9．52）和假手术组（42．43±6．77、40．32±6．48、44．10±9．36）明显升高（均P〈0．05）；且脓毒症组随病情加重PPARγ活性逐渐下降，IL-6于24h达高峰后下降，组内各时间点间两两比较差异均有统计学意义（均P〈0．05）。脓毒症大鼠12h时有核细胞PPARγ活性与血浆IL-6水平呈显著负相关（r=-0．703，P=0．023）。结论脓毒症有核细胞PPARγ活性下降，血浆促炎介质IL-6升高，且两者呈负相关。

Objective To observe the-relationship between activity of peroxisome proliferator activated receptor γ （PPARγ） in nucleated cell and level of pro-inflammatory mediator interleukin-6 （IL-6） in plasma of rats with sepsis. Methods According to the random number table, 90 male Sprague-Dawley （SD） rats were randomly divided into three groups, namely control group, sham operation group and sepsis group. Each group was further divided into three subgroups according to postoperative time points, i.e. 12, 24 and 48-hour subgroups. Each subgroup consisted of 10 rats. Sepsis was reproduced by cecal ligation and puncture （CLP）. The PPARγ activity in nucleated cells and IL-6 level in plasma were detected by enzyme-linked immunosorbent assay （ELISA）. Results The PPARγ activity in nucleated cells was significantly decreased at 12, 24 and 48 hours in sepsis group （A value： 0. 279±0. 004, 0. 264±0. 009, 0. 245±0. 012） compared with control group （0. 292 ± 0. 007, 0. 293 ± 0. 004, 0. 293 ± 0. 005） and sham operation group （0. 295 ± 0. 008, 0. 295±0. 006, 0. 294± 0. 007）, while the IL-6 level was significantly increased in sepsis group （ng/L： 365.25 ± 15.53, 507.16 ± 20. 86, 437.89 ± 25.09） compared with control group （43. 54 ± 11.10, 48. 82±10. 62, 42.96±9.52） and sham operation group （42.43±6.77, 40. 32±6.48, 44. 10±9. 36, all P〈 0. 05）. When septic condition became worse, the PPARγ activity in nucleated cells of sepsis group lowered, and IL-6 level was gradually elevated alter operation, reaching the peak at 24 hours, and then gradually lowered, and the difference of the value between any two time points was all statistically significant （all P〈0. 05）. There was a negative correlation between the PPARγ activity in nucleated cells and IL-6 level in 12-hour subgroup of sepsis group （r=-0. 703, P=0. 023）. Conclusion In septic rats, the PPARγ activity in nucleated cells was lowered while the pro-inflammatory mediator IL-6 level in plasma elevated, and there was a negative correlation between PPARγ activity and IL-6 level.