摘要
采用双向电泳联用MOLDI-TOF-TOF质谱对水稻感病品种武育粳3号和抗病品种KT95-418感染水稻条纹病毒(Rice stripe virus,RSV)前后的叶片进行蛋白质组学分析。结果显示,RSV基因组编码的病害特异蛋白(disease specificprotein,SP)在武育粳3号中的积累量明显高于KT95-418中。其他25个蛋白经质谱成功鉴定,包括RSV NS2蛋白,寄主中与光合作用、细胞氧化还原状态和离子平衡状态及蛋白的合成、转运与翻译后修饰等相关的蛋白。对这些差异表达的蛋白与水稻感、抗病的作用进行了分析。
Two dimensional electrophoresis（2-DE） and MOLDI-TOF-TOF MS were used to analyse the proteomic changes in leaves of the susceptible cultivar WuYu3 and resistant cultivar KT95-418 rices infected with Rice strip virus（RSV）.The results showed that disease specific protein（SP） of RSV accumulated more in susceptible cultivar WuYu3 than that in resistant cultivar KT95-418.The other 25 proteins were identified successfully by MS,including RSV NS2,host proteins related to photosynthesis,dynamic balance of cellular redox state or ions and protein translation/translocation or modification.The possible functions of these host proteins in the susceptible and resistant cultivars were discussed.
出处
《植物病理学报》
CAS
CSCD
北大核心
2011年第3期253-261,共9页
Acta Phytopathologica Sinica
基金
国家转基因生物新品种培育重大专项重点课题(2009ZX08009-044B)
国家自然科学基金资助项目(30770090)
农业部农业公益性行业科研专项(nyhyzx07-051)
