血管紧张素原M235T基因突变与原发性高血压关联的研究

The association study of M235T allele of angiotensinogen and essential hypertension

目的 :研究血管紧张素原 ( AGT)基因第二外显子 M2 35 T等位基因的变异对血压的影响。方法 :应用多聚酶链反应 ( PCR)结合限制性酶切方法对 10 5例健康体检者与 10 2例原发性高血压 ( EH)患者进行基因突变的检测。结果 :EH患者 T2 35等位基因频率 ( 0 .446)高于对照组( 0 .32 3) ,P <0 .0 5。在男性 EH患者与男性对照组中差别更为明显 ( P <0 .0 1)。结论 :AGT基因的突变与 EH的发病具有相关性。对男性 EH影响可能更大。

Objective:To study the association between M235T allele mutation in exon 2 of angiotensinogen(AGT) gene and essential hypertension(EH).Method:Polymerase chain reaction combined with restrict enzyme digestion was used to detect the target gene variation in 105 normotensive controls and 102 hypertensive patients.Result:T235 allele frequency was significantly higher in hypertensive patients than in controls( 0.446 vs 0.323 ,P< 0.05 ) and it was noticeably higher in male hypertensives.Conclusion:The M235T allele variation was associated with EH,especially in male.