摘要
采用多聚酶连反应(PCR)扩增人IL—3cDNA片段,以地高辛(Digoxigenin—11—dUTP)为标记物,随机引物法标记IL—3cDNA探针。探针经斑点杂交和核酸原位杂交证明:用制备IL—3cDNA探针检测细胞IL--3mRNA表达具有特异性强,灵敏度高,使用简便,杂交结果直观,探针可较长时间保存和无放射性污染等优点。
By using the techniques of polymerase chain reaction(PCR),human IL - 3cDNA probe was prepared with pUC18- IL- 3 plasmid, labeled with digoxigenin - 11dUTP and identified with dot blot. We detected the expressionof IL- 3 mRNA in murine bone marrow stromal cells, human fetal thymocytes and splenic cells by nucleisitu hybridization. The results indicated that the prepared IL-3cDNA probe can provide the sensitivitysimplicity of handling needed to replace radioactiassays.
出处
《重庆医科大学学报》
CAS
CSCD
1999年第4期337-340,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金!8967088
重庆市中医药管理局资助课题