甲基斑蟊胺诱导HepG_2细胞凋亡的实验研究

Apoptosis of human HepG_2 induced by N-methylcantharidinimidum

为探讨甲基斑蟊胺对ＨｅｐＧ２细胞凋亡的诱导作用，揭示其抗癌机制，用ＭＴＴ法观察药物对细胞的生长抑制作用，用ＴＵＮＥＬ法、流式细胞仪及光镜等技术研究癌细胞凋亡。结果表明药物对癌细胞有明显的抑制生长作用；光镜可见染色质浓缩、核碎裂等改变；流式细胞仪可检测到凋亡峰；６ｍｇ／ｍｌ药物作用５天，ＴＵＮＥＬ法强阳性。提示甲基斑蟊胺可诱导ＨｅｐＧ２细胞凋亡。

To demonstrate the induction of apoptosis of human HepG_2(Hepatocellular Carcinoma) cell lineN-methylcantharidinimidum in order to investigate the mechanism of its anticancer action. MTT method,TUNIflow cytometry(FCM) and light microscopy were used. HepG2 cells treated with N-ethylcantharidinimidumunderwent cell proliferation inhibition;typical nuclear condensation and fragmentation can be detected by lightmicroscopy;apoptotic peaks were also detected on FCM,and the TUNEL-positive cells can be seen after beingtreated with N-methylcantharidinimidum at 6mg/ml for 5days. N-methylcantharidinimidum can induce apoptosisof HepG2 cells in a certrain concentration.