骨保护蛋白和Wnt-1在张力侧牙周组织中的表达

Osteoprotegerin and Wnt-1 express in tension side of periodontal tissue

目的运用RT-PCR和免疫组织化学方法,观察骨保护蛋白(OPG)和Wnt-1在大鼠正畸牙牙周组织改建过程中张力侧的表达,探讨OPG和Wnt-1与正畸牙周组织改建的关系。方法制备80只大鼠正畸牙移动模型,牙移动1、3、5、7 d分别取材,苏木精-伊红染色,观察大鼠张力侧牙周组织的形态变化,然后以RT-PCR和免疫组织化学方法检测张力侧牙周组织中OPG和Wnt-1的表达。结果在牙移动3 d后,OPG表达在张力侧增强,5 d达到高峰,7 d时下降,免疫组织化学与RT-PCR检测结果一致;免疫组织化学显示,Wnt-1在3、5d呈阳性,但在RT-PCR中未检测出。结论 OPG参与了正畸牙周组织的改建,OPG在张力区随正畸牙移动的表达升高具有时间依赖性;Wnt-1与正畸牙周组织改建的相关性尚无定论,需进一步研究。

Objective Using immunohistochemistry and RT-PCR two detection methods,We aimed to investigate the changes of osteoprotegerin（OPG） and Wnt-1 expression in tension side by periodontal tissue during orthodontic tooth movement in rats and detect the relationship between OPG,Wnt-1 and orthodontic periodontal tissue remodelling.Methods Orthodontic tooth movement model of 80 merely rats was prepared,and at the 1,3,5,7 d after tooth movement the teeth were extracted respectively.The hematoxylin-eosin staining of rats tension side of the morphology of periodontal tissue was observed,and the RT-PCR and immunohistochemical method was used to detect Wnt-1 and OPG expression in tension side periodontal tissue.Results The expression of OPG were weak in normal periodontal tissue.In tension area,OPG expression increased in 3 days,then reached the maximum at day 5,and new bone formation could be seen at that time;on day 7,OPG expression declined.There was no Wnt-1 expression in RT-PCR,but on day 3,5,Wnt-1 expression positive cells of the periodontal ligament cell in immunohistochemical method.Conclusion OPG participated in the process of orthodontic periodontal tissue rebuilding,and the OPG expression increased in tension area in a time-dependent manner;the correlation of Wnt-1 with orthodontic periodontal tissue reconstruction is not certain,need to be studied further.