甲型H1N1流感患者Th1和Th2型细胞因子的检测及其意义

Quantitative detection of type Th1 and type Th2 cytokines in the patients with type A H1N1 influenza

目的探讨甲型H1N1流感患者外周血Th1和Th2细胞因子的表达水平,为临床诊断H1N1感染及判断疾病的严重程度提供一定的客观依据。方法采用流式细胞仪检测24例甲型H1N1流感患者和24名健康者外周静脉血Th1、Th2细胞因子,对结果进行统计学分析。结果 H1N1甲流患者与健康对照组相比,IL-6、IL-10水平差异有统计学意义(P<0.05),IL-2I、L-4、TNF-α和IFN-γ差异无统计学意义(P>0.05);重症组与一般患病组比较IL-6水平差异有统计学意义(P<0.05),IL-2I、L-4I、L-10、TNF-α、IFN-γ水平差异无统计学意义(P>0.05)。结论与健康者相比,甲型流感患者的Th2型细胞因子水平显著升高,IL-6、IL-10,特别是IL-6可能在流感发病中发挥作用。

Objective To detect the concentrations of types Th1 and Th2 cytokines in the serum samples of patients with type A influenza and provide a reference for diagnosis of this disease and determination of its severity. Methods The concentrations of types Th1 and Th2 cytokines in the serum samples of 24 patients with type A influenza were detected by flow cytometry and compared to those of 27 healthy persons as a control. Statistical analyses were carried out to determine significant differences between the groups. Results Compared to the control group, the serum levels of type Th2 cytokines IL-6 and IL-10 were significantly higher（P〈0.05）in patients with influenza, while levels of the cytokines IL-2, IL-4, TNF-α and IFN-γ did not differ between patients and the control group. The level of IL-6 level was also significantly higher in patients with severe flu than in regular patients（P〈0.05）, but levels of the cytokines IL-2, IL-4, TNF- α, and IFN-γ did not differ between the two patient groups. Conclusion The concentrations of Th2 cytokines in the serum samples of patients with type A influenza are significantly higher. IL-10 IL-6 in particular are closely related to the pathogenesis and severity of type A H1N1 influenza.