两种方法建立多囊卵巢综合征大鼠模型的实验研究

Comparison of polycystic ovary syndrome models induced by dehydroepiandrosterone and progesterone in combination with human chorionic gonadotrophin in SD rats

目的:探讨脱氢表雄酮(DHEA)法与孕激素联合人绒毛膜促性腺激素(hCG)法建立的幼年雌性SD大鼠多囊卵巢综合征(PCOS)动物模型效果。方法:模型组Ⅰ44只SD大鼠用孕激素和hCG建模;模型组Ⅱ44只大鼠用DHEA建模,观察两组模型的稳态模型评估(HOMA)指数、其他主要指标及卵巢形态学改变,并与对照组Ⅰ、Ⅱ比较。结果:模型组Ⅰ和模型组Ⅱ失去规律动情周期的大鼠的比例分别为86.4和77.3;模型组Ⅱ体重低于对照组(P<0.05),血清E2高于对照组;两建模组大鼠血清睾酮、空腹血清胰岛素、空腹血糖和HOMA指数均高于对照组(P<0.05);两模型组大鼠卵巢均可见多个扩张的囊泡。结论:两法制作的PCOS大鼠模型均获得成功,孕激素联合hCG法更为理想。

Objective： To compare polycystic ovary syndrome （PCOS） models induced by dehydroepiandrosterone （DHEA） and progesterone in combination with human chorionic gonadotrophin （hCG） in SD female rats. Methods ： Forty - four SD rats in Model group I were given progesterone in combination of hCG, and 44 SD rats in Model group II received DHEA. The normal rats served as the control. The main outcome measures included vaginal epithelial cells in smears, serum estrogen, total testosterone, progesterone levels, fasting blood insulin and glucose, homeostasis model assessment （HOMA） index and ovarian morphological changes. Results： The rates of rats lost of menstrual cycle regularity were 86.4% and 77.3% in Model group I and II respectively. The body weight in DHEA - induced group were lower than that in the control group （ P 〈 O. 05 ）. Serum E2 level in DHEA - induced group was higher than that in the control group （ P 〈 O. 05 ）. The serum testosterone, fasting insulin, fasting blood glucose levels and the HOMA index were significantly higher in model groups than those in the control group （P 〈 0.05 ）. The ovaries in both model groups showed multiple follicular cysts. Conclusion： The PCOS rat model could be successfully induced by two methods, and the method using progestrone in combination with hCG may be more ideal in thi,~ ~tHdv.