短梗胡枝子的组织培养研究

Tissue Culture of Lespedeza cyrtobotrya

[目的]研究短梗胡枝子(Lespedeza cyrtobotrya)的组织培养。[方法]以短梗胡枝子种子为材料,对其组织培养进行了系统研究。[结果]2.1%次氯酸钠灭菌8 min,发芽率较高,且污染率为0;在初代培养中,基础培养基为MS,6-BA对分化系数影响达到显著水平,适宜的增殖培养基为MS+1.00 mg/L6-BA+0.10 mg/LNAA+0.01 mg/L2,4-D,分化系数可达6.69;在继代培养中,适宜的培养基为MS+1.00 mg/L6-BA+0.05 mg/L2,4-D;在生根培养中,IBA浓度为0.50 mg/L时,生根率和平均生根数都最佳。[结论]为进一步开展胡枝子基因工程遗传改良提供了理论依据。

[Objective]The aim was to carry out study on tissue culture of Lespedeza cyrtobotrya.[Method]The seeds of L.cyrtobotrya were used as materials to study on its tissue culture.[Result] The best disinfectant time to L.cyrtobotrya seeds was 8 min with 2.1% NaClO,in which shooting percent reached 37.8% and no polluted situations occurred.In the primary culture with the MS as basal medium,the concentration of 6-BA showed a significant effect on the index of buds differentiation,the optimum differentiation culture medium was MS＋1.00 mg/L 6-BA ＋0.10 mg/L NAA＋0.01 mg/L 2,4-D,on which the index of generation could reach 6.69.The optimum secondary differentiation culture medium was MS＋1.00 mg/L 6-BA＋0.05 mg/L 2,4-D.The plants can generate the highest roots and rooting percent with IBA 0.50 mg/L.[Conclusion] This study had provided theoretical basis for genetic improvement of L.cyrtobotrya.