高效液相色谱-紫外检测法测定水产品中组胺的含量

Determination of Histamine in Fishery Products by HPLC-UV Method

[目的]建立一种测定水产品中组胺含量的高效液相色谱-紫外检测法。[方法]用高氯酸提取水产品中的组胺,丹磺酰氯衍生后,用配有紫外检测器的高效液相色谱仪进行测定,外标法定量。[结果]组胺在0.5~20.0μg/m l范围内线性关系良好,相关系数r〉0.999。空白样品中组胺添加浓度在50~1 000 mg/kg时,平均回收率在81.0%~94.8%,批次内和批次间相对标准偏差均〈10%。组胺的检出限为25 mg/kg,定量限为50 mg/kg。[结论]该方法操作简便,能够满足水产品中组胺含量的测定要求。

[Objective] The aim was to construct a method for the determination of histamine in fishery products by HPLC-UV.[Method] Histamine in the test sample was extracted with perchloric acid,and then derived by dansyl chloride,and nextly determined by high performance liquid chromatograph with UV detector,at last quantified by an external standard mode.[Result] The histamine showed a good linear relationship within the range of 0.5-20 μg/ml with the correlation coefficient r 0.999.The average recoveries ranged from 81.0% to 94.8% and the relative standard deviation was all below 10% when the addition concentration of histamine in the blank sample was within 50-1 000 mg/kg.The detection limit of histamine was 25 mg/kg and the limit of quantification was 50 mg/kg.[Conclusion] The sample was easy to operate,which could satisfy the requirements of the determination of histamine in fishery products.