4,9-二[N-(2-二甲氨基)乙基]-9-吖啶胺-4-甲酰胺与蛋白质相互作用

Interaction between 4,9-di[N-(2-dimethylamino)ethyl]-9-Aminoacridine-4-Formamide and Protein

应用水溶性4,9-二[N-(2-二甲氨基)乙基]-9-吖啶胺-4-甲酰胺(DNAF)探针,研究了DNAF与牛血清白蛋白(BSA)的相互结合作用机理。计算出相应的热力学参数ΔH、ΔG以及ΔS;通过荧光猝灭方法研究结合作用,测定出结合位点数n和表观结合常数KA;根据荧光共振能量转移理论求得供体BSA和受体DNAF的距离r;研究了DNAF对蛋白质内源荧光猝灭的猝灭机制和主要作用力类型。

Mutual combination mechanism of DNAF with bovine serum albumin（BSA） was investigated by applying water-soluble 4,9-di[N-（2-dimethylamino） ethyl]-9-aminoacridine-4-formamide（DNAF） probe.The corresponding thermodynamic parameters ΔH,ΔG and ΔS were calculated and through fluorescence quenching method,the number of binding sites n and apparent binding constant KA were determined.The distance of donor BSA and receptor DNAF,namely r was derived based on the theory of fluorescence resonance energy transfer.The quenching mechanism of DNAF on intrinsic fluorescence quenching of protein and the types of interaction forces were proposed.