摘要
NPR1(Non-expresser of pathogenesis related genes 1)是水杨酸(Salicilic acid,SA)介导的系统获得抗性(Sys-temic aquired resistance,SAR)的关键调控因子,在水稻中过量表达拟南芥NPR1和水稻NH1/OsNPR1(NPR1 homo-logue 1)(NPR1的同源物)可增强抗病性。水稻基因组中还有4个NPR1旁系同源物(Paralog),为NH1的家族蛋白。本试验利用双分子荧光互补技术(Bimolecular Fluorescence Complementation,BiFC,or split YFP)研究了水稻NH1家族蛋白之间在活体内的相互作用,发现除NH2外,NH1、NH3、NH4和NH5都会和自身的蛋白互作,产生荧光信号,并且它们还会和家族成员的其他蛋白相互结合产生荧光信号。
NPR1（Non-expresser of pathogenesis related genes 1） is the master regulator of salicylic acid-mediated systemic acquired resistance.Over-expression of Arabidopsis NPR1 and rice NH1（NPR1 homolog1）/OsNPR1 in rice results in enhanced resistance.There are four rice NPR1-paralogs in the rice genome,they are family members of NH1.Protein-protein interaction between members of NH1 family were studied in vivo by bimolecular fluorescence complementation assay（BiFC,or split YFP）.NH1,NH3,NH4,and NH5 all can interact with itself to generate fluorescence signals.They can also interact with other members in the family.But NH2 has no interaction with anyone.
出处
《华北农学报》
CSCD
北大核心
2011年第2期30-34,共5页
Acta Agriculturae Boreali-Sinica
基金
美国农业部USDANRIgrant(#2004-63560416640)
关键词
水稻
NH1家族蛋白
双分子荧光互补试验
蛋白互作
Rice
NH1 family members
Bimolecular fluorescence complementation assay
Protein-protein interaction
