期刊文献+

猪Reg3基因的克隆与序列分析

Molecular Cloning and Sequence Analysis of Porcine Gene Reg3
下载PDF
导出
摘要 在猪的肠道组织中克隆Reg3基因,提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化E.coliJM109,检测阳性克隆、测序并进行序列分析。结果表明:克隆的猪Reg3基因与人、绵羊、马的同源性分别为83.7%,82.3%,82.2%;克隆了猪Reg3全长基因并注册GenBank注册号为Accession.FJ531494。 In order to clone and analyze the gene Reg3 in intestinal canal tissues of pig,the total RNA was extracted and mRNA sequence of Reg3 gene was amplified by RT-PCR method which used two designed primers.The PCR productswere ligated into the pMD-19T vector,and then transformed into competent cells of E.coli JM109.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the Reg3 nucleotide sequence shared 83.7%,82.3% and 82.2% homology with that of human,sheep and horse respectively,revealing porcine Reg3 gene had been successfully cloned in the present study.The sequence has been submitted to GenBank(Accession FJ531494).
出处 《华北农学报》 CSCD 北大核心 2011年第2期55-57,共3页 Acta Agriculturae Boreali-Sinica
基金 国家科技攻关计划子课题(2006BAD04A03-10)
关键词 Reg3基因 克隆 序列分析 Pig Reg3 gene Molecular cloning Sequence analysis
  • 相关文献

参考文献4

二级参考文献16

  • 1Min-Jie Luo Mao-De Lai Department of Pathology,School of Medicine,Zhejiang University,Hangzhou 310031,P.R.China.Identification of differentially expressed genes in normal mucosa,adenoma and adenocarcinoma of colon by SSH[J].World Journal of Gastroenterology,2001,7(5):726-731. 被引量:22
  • 2马华,梅峰,马千里,张红军,周德山.RegⅣ在胃癌中的表达及其临床意义[J].第三军医大学学报,2006,28(5):441-443. 被引量:7
  • 3Martin H Bluth,Sameer A Patel,Brian K Dieckgraefe,Hiroshi Okamoto,Michael E Zenilman.Pancreatic regenerating protein (regⅠ) and regⅠreceptor mRNA are upregulated in rat pancreas after induction of acute pancreatitis[J].World Journal of Gastroenterology,2006,12(28):4511-4516. 被引量:19
  • 4Terazono K, Yamamoto H, Takasawa S, et al. A novel gene activated in regenerating islets. J Biol Chem, 1988,263 : 2111 -2114.
  • 5Liu JL, Cui W. Which gene, Reg2 or Reg3beta, was targeted that affected liver regeneration? Hepatology, 2007, 45 : 1584 - 1585.
  • 6Nata K, Liu Y, Xu L, et al. Molecular cloning, expression and chromosomal localization of a novel human REG family gene, REG Ⅲ. Gene, 2004, 340 : 161 - 170.
  • 7Castellarin ML, Petropavlovskaia M, Lipsett MA,et al. The identification and sequence analysis of a new Reg3gamma and Reg2 in the Syrian golden hamster. Biochim Biophys Acta, 2007, 1769 : 579 - 585.
  • 8Ose T, Kadowaki Y, Fukuhara H, et al. Reg I-knockout mice reveal its role in regulation of cell growth that is required in generation and maintenance of the villous structure of small intestine. Oncogene, 2007, 26 : 349 - 359.
  • 9Takasawa S, Ikeda T, Akiyama T, et al. Cyclin D1 activation through ATF-2 in Reg-induced pancreatic beta-cell regeneration. FEBS Lett, 2006, 580 : 585 -591.
  • 10Lipsett M, Hanley S, Castellarin M, et al. The role of Islet Neogenesis-Associated Protein (INGAP) in islet neogenesis. Cell Biochem Biopbys, 2007, 48 : 127 -137.

共引文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部