苏丹红Ⅰ和对位红残留检测ELISA试剂盒的研制

Development of Competitive Diagnostic ELISA Kit for Rapid Detection of Sudan Ⅰ and Para Red Residues

针对苏丹红I和对位红的芳香胺共同化学结构特点,采用琥珀酸酐法人工合成苏丹红I免疫原,建立了快速检测苏丹红I和对位红残留的酶联免疫方法。同时对其灵敏度、准确度、特异性、基质效应性等技术指标进行测定。结果表明:该试剂盒与其它系列苏丹红均无交叉反应;试剂盒检测相关系数R2=99.56%,试剂盒最低检出限为0.1μg/L,半数抑制率IC50=0.599μg/L;油基质和水基质的辣椒样品的平均添加回收准确率分别为79.9%和78.2%;不同基质对该试剂盒的检测结果影响不大。该试剂盒适合于苏丹红I和对位红残留的快速检测,具有较高的推广价值。

Based on the common structure of aromatic amines in Sudan Ⅰ and Parared,Sudan Ⅰ immunogen was synthesized by succinic anhydride method.A competitive ELISA test kit for detection of Sudan Ⅰ and Parared was developed with antiSudan Ⅰ monoclonal antibody（Sudan Ⅰ-mAb） and its sensitivity,accuracy,specificity and the effect of matrix were tested,respectively.Moreover,the Pepper was detected with this ELISA-Kit.The results indicated that the Sudan Ⅰ-Kit had little or no cross-reactivity with other analogues;the correlation coefficient R2 was 99.56%,the lowest detection limit was 0.1μg/L,the IC50 was 0.5999 μg/L and the detection limit was 8 μg/ L.The recoveries of Sudan I spiked in Pepper was 79.9% and 78.2%.The matrix had no effect on the results of the Sudan I-Kit.The Sudan Ⅰ-Kit was proved to be useful in the rapid test of Sudan Ⅰ and Para red residues in food.