兔出血症-巴氏杆菌病二联灭活苗的研制

Study on Bicombinant Inactivated Vaccine of Rabbit Heamorrhagic Disease and Pasteurellosis

筛选出了具有良好免疫原性的兔瘟强毒株GMH881和兔巴氏杆菌C51-17株,研制出了兔出血症-巴氏杆菌二联灭活苗,对疫苗进行了免疫剂量、免疫产生期、兔出血症抗体测定、免疫保护期、保存期等试验：确定出二联苗免疫剂量1.0 mL;二联苗在免疫后5 d对兔病毒性出血症强毒的保护率达到100%,二联苗免疫后7d对兔巴氏杆菌的保护率均达到80%以上;二联苗的兔病毒性出血症抗体测定,结果表明,二联苗免疫家兔后7d HI抗体均可达22.75,15 d明显上升,二联苗HI效价30 d达到最高峰,可达28.0,二联苗在60~120 d均可维持在27.0~25.5水平,到180 d时抗体水平面略有下降为25.25,二联苗分别在免疫后4个月、6个月用兔出血症强毒攻击均产生100%保护,在6个月用2个MLD的兔巴氏杆菌攻击保护率可达70%以上,二联苗4~8℃条件下保存期暂定为1年,25℃条件下暂定为半年。

Rabbit hemorrhagic disease virulent strain GMH881 and pasteurellosis strain C51-17 were selected as good immunogenicity,bicombinant inactivacted vaccine were developed successfully.To ascertain the safety and immune effects of bicombintaint invactivated vaccine,test was carried out on immune dosage,potency,immune duration and storage life.Immune dose of the bicombintaint inactivated vaccines was 1.0 mL,the immune protection rate of bicombintaint invactivated vaccine against RHD was 100 % on the 5th day vaccination,the immune protection rate of bicombintaint invactivated vaccine against Pm was over 80 % on the 7th day after vaccination.To supervise the level of RHDV through HI method,the results showed that the titers of bicombinant inactivated caccine were 22.75 after 7 d of injection,and the antibody effect could reach very high level after 15th day,which could reach peak level（28.0） on day 30,antibody effect level of bicombinant inactivated vaccine was 27.0~25.5 during 60 ～ 120 days,on day 180 it slightly reduced to 25.25.Four and six month after vaccination,the protection rate of the bicombintaint inactivated vaccines was 100% against rabbit haemorrhagic disease with injecting pathogens,more than 70% against pasteurellosis with 2 MLD of the pasteurlla multocida strain C51-17 on 6th month.The bicombintaint invactivated vaccine can provide effective protection when it was stored at 4 ～ 8℃ for 12 months,at 25 ℃ for 6 months.