FHIT基因在胃癌中的甲基化表达及其与胃癌临床病理特征的关系

Methylation of fragile histidine triad gene in gastric cancer and its association with helicobacter pylori

目的探讨胃癌(GC)组织中脆性组氨酸三联体(FHIT)基因的甲基化表达及其与胃癌临床病理特征的关系。方法选取我院胃镜检查诊断为胃癌的74例患者及36例慢性胃炎患者为研究对象。应用甲基化特异性PCR(MSP)检测两组胃黏膜中FHIT基因的甲基化状态,对比相应的病理特征并进行分析。结果胃癌组与对照组组织中FHIT基因甲基化阳性率分别为51.4%及5.6%,胃癌组FHIT基因甲基化阳性率显著高于对照组(P<0.05),且甲基化表达与GC患者的年龄、性别、肿瘤大小、部位及组织学类型无关(P>0.05),与病变的浸润深度、淋巴结转移和远处转移有关(P<0.05)。结论 FHIT基因甲基化修饰参与胃癌的发生发展过程,可反映胃癌侵袭转移的程度。

Objective To investigate the methylation of fragile histidine triad（FHIT） gene and explore possible relationship between FHIT methylation and clinicopathological features of gastric carcinoma（GC）.Methods 74 cases with GC and 36 cases with chronic gastritis diagnosed by gastroscopy were selected as study cases.Methylation specific PCR（MPC） was performed to test the methylation status of FHIT gene in the gastric mucosa of the two groups.The corresponding clinicopathological features between them was also contrast analyzed.Results The methylation rate of FHIT in tissues from GC group and control group was 51.4% and 5.6% respectively.The methylation rate of FHIT in GC was significantly higher than that in control group（P〈0.05）.FHIT methylation was related to the depth of invasion,metastasis in lymph node and metastasis in distance（P〈0.05）,while it has no relationship with age,gender,tumor size,tumor location and histological type.Conclusion Methylation of FHIT gene takes part in the progression of GC and it can reflect the degree of invasion and metastasis in GC.