神经干细胞和神经细胞的拉曼光谱分析

Raman spectral analysis of NSCs and NCs

从光谱学角度研究神经干细胞(NSCs)与神经细胞(NCs),是一个新的干细胞研究方法,能够为临床应用提供一定的实验参考依据。本文通过体外培养NSCs和NCs,应用激光共焦显微拉曼光谱仪测定两种细胞的拉曼光谱。实验结果显示,NSCs的拉曼峰646 cm-1,2917 cm-1与NCs的拉曼峰652 cm-1,2908 cm-1处存在较大差异。NCs核中B型DNA胸腺嘧啶和酪氨酸含量相对更丰,而NSCs核中具有NO2成分而NCs核中较少或没有。NSCs比NCs的DNA、酪氨酸、蛋氨酸、腺嘌呤、苯基丙氨酸、胡萝卜素、酰胺Ⅲ等含量更丰,其中以胡萝卜素和酰胺Ⅲ的相对含量最高。本文从光谱学角度分析NSCs与NCs的光谱区别,以为临床应用提供一定的实验参考依据。

Spectroscopy is a new technique to study stem cells.It can provide a certain reference for clinical application.We cultured NSCs and NCs in vitro respectively and achieved their Raman spectra using a laser confocal microscope Raman spectrometer.The results show that there is difference between 646 cm-1,2917 cm-1of NSCs and 652 cm-1,2908 cm-1of NCs.There are more thylakoid of DNA and casein in NCs nuclei while NSCs nuclei has some NO2 which does not exist in NCs.NSCs seems to contain more DNA,tryptophan,adenine,carotenoid,amideⅢ etc than NCs.This paper analyzes difference of Raman spectra of neural stem cells and neural cells in spectroscopy terms in order to provide some experimental reference for clinical application.