荧光法同时测定对硝基酚和对氨基酚

Simultaneous Determination of p -Nitrophenol and p -Aminophenol by Fluorimetry

在ｐＨ１０～３０的ＨＣｌ介质中，荧光物质对氨基酚的激发波长和发射波长分别为２７１ｎｍ和３７１ｎｍ．当有对硝基酚共存时，用硼氢化钠将其还原为对氨基酚，可用荧光光度法同时测定两者的含量．对硝基酚和对氨基酚的检测限分别为０２７μｇ／ｍＬ和０３２μｇ／ｍＬ．样品加标回收率分别为对氨基酚：９５１％～１０１５％，对硝基酚：８９８％～１００５％．

In a medium of HCl (pH1 0～3 0),the excitation and emission wavelengths of fluorescence spectrophotometer for p -Aminophenol are 271 nm and 371nm,respectively.When p -Nitrophenol exists in it, p -Nitrophenol can be reduced into p -Aminophenol by adding NaBH4,the amount of two components can be determined simultaneously by fluorimetry.The detection limit of the method is 0 27μg/mL for p -Nitrophenol and 0 32μg/mL for p -Aminophenol.The recovery of standard addition is from 95 1% to 101 5% for p -Aminophenol and from 89 8% to 100 5% for p -Nitrophenol.