大豆含水量和粉碎细度对索氏抽提法测粗脂肪含量的影响

Influence of soybean moisture content and smash degree on crude fat content tested by Soxhlet extraction

目的讨论索氏抽提法检验大豆粗脂肪含量时,应该如何处理大豆样品。方法对不同粉碎细度、不同水分含量的大豆样品依据索氏抽提法测定粗脂肪含量。结果使用无水乙醚抽提时,样品粉碎细度对最终结果没有影响,样品不烘干影响抽提效果,使用石油醚抽提时,样品含水量对最终结果没有影响,样品粉碎细度影响抽提效果。结论在使用索氏抽提法测定大豆粗脂肪含量时,样品应该使用高速万能粉碎机粉碎完全,并烘干30分钟以上,以保证最佳抽提效果。

Objective Discuss how to deal with the soybean samples by Soxhlet extraction to test crude fat content of soybean.Methods Test crude fat content of different moisture content and smash degree of soybean samples by Soxhlet extraction.Results Extracted with anhydrous ether,sample smash degree has no influence on final results,and extraction effects will not be affected by drying.Extracted with petroleum ether,sample water content has no influence on final results,and smash sample will affect extraction effects.Conclusion When test crude fat content by Soxhlet extraction,samples should be totally smashed by high-speed grinder,and dry above 30 minutes to guarantee best extraction results.