大鼠杏仁核电点燃癫痫模型的建立及海马病理变化的研究

Establishment of Epilepsy Kindling Models of the Rat's Amygdala by Electrical Stimulation and Study of the Pathological Changes of Hippocampus

目的:探讨建立大鼠杏仁核电点燃癫痫模型的方法及海马的病理学变化。方法:利用立体定向手术,向Wistar大鼠杏仁核(前囟后2.2 mm、旁开4.8 mm、颅骨平面下深8.5 mm)植入微电极。术后7 d予以电刺激(50Hz、0.8mA),观察点燃过程中大鼠行为学表现及脑电表现。HE染色、GFAP免疫组化染色,分析大鼠海马及杏仁核区的病理学改变。结果:点燃率为88.6%。大鼠在0.8mA刺激电流强度下,(19.89±3.64)d可以达到点燃要求。海马的病理改变为:海马区锥体细胞神经元缺失及神经胶质细胞的增生。结论:杏仁核靶点参数进行立体定向手术植入电极是可靠的,单侧杏仁核在频率50Hz、0.8mA细电流刺激下,可以达到点燃要求。点燃模型的大鼠行为学表现、脑电表现及海马病理学改变与人类颞叶内侧型癫痫相似。

Objective To explore a better method to establish an epilepsy kindling model of the rat＇ s amygdala by electrical stimulation and pathological changes of hippocampus. Methods The Wistar rat was implanted the microeleetrical bipolar into the right amygdala by stereosurgery. The operated rats were given electrical stimulus 7 days after surgery. The rat~ behavior and the appearance of the frontal cortext EEG was monitored by Videotape recording and the Taimeng EEG recording system during the kindling process. At last, we took out the rat~ brain to make paraffin section and analyzed pathological changes of the hippoeampus and amygdala by HE stain and GFAP immunohistological stain. Results ：The rate of kindling was 88.6%. The mean time about the rat fully kindled under the 0.8 milliampere electric current were 19.89 days and the standard deviation were 3.64 days. The changes found in the tissue slices were the loss of hip- poeampus neurons in stratum pyramidale and the hyperplasia of glia cell. Conclusion It is reliable to implant the microelectrical bipolar into the basolateral nucleus of the amygdala according to the target site. The stimulation （0.8 milliampere, 50Hz square wave, minute current flow） can kindle the amygdala. The behavioral performance ,EEG manifestations and pathological changes of the hippocampus of the kindled rat resemble human mesal temporal lobe epilepsy.