氢化物-无色散原子荧光光谱法测定血液中的硒

Determination of Selenium in Human Blood by Hydride Generation-Atomic Fluorescence Spectrometry

采用氢化物－原子荧光光谱法测定人体血液中硒的含量．健康者（30人）血液中硒的平均含量为114．6±11．2ng·mL^（－1）；肿瘤及心脏病患者（20人）血液中硒的平均含量为77．4±11．1ng·mL^（-1）。研究结果表明：肿瘤及心脏病患者血液中硒的含量明显低于正常人．该方法的回收率为90．5％－107．0％，相对标准偏差为6．6％，检出极限为0．65ng·mL（-1）．

The determination of selenium in human blood by hydride - generation - atomic fluoresecence spectrometry (HG -AFS) is studied. HNO_3 - HClO_4 - HCl is used for sample pretreatment. The recoveries are from 90. 5% to 107. 0%, the detection lindt is 0. 65ng·mL^(-1), and the relative standard deviation (RSD) is 6.6%, Under optimized experimental conditions, selenium in human blood has been determined, The average concentration of Se is 114.6±11 .2ng·mL^(-1) for thirty healthy people, and 77 .4 ±11. 2ng·^(-1) for twenty patients. The resultS showed that the concentrafion of Se in the blood of a patient is lower than tha in a healthy person, and can be used for the clinical diagnosis.