三七总皂苷对慢性马兜铃酸肾病大鼠体内抗氧化作用的研究

Research on antioxidation of panax notoginseng saponins in rats of chronic aristolochic acid nephropathy

目的观察三七总皂苷对慢性马兜铃酸肾病损害大鼠模型抗氧化作用。方法 Wistar大鼠随机分为正常对照组、模型组及三七总皂苷低、中、高剂量组。造模大鼠按马兜铃酸20 mg.kg-1.d-1的剂量灌服关木通浸膏,1周后改为按马兜铃酸15 mg.kg-1.d-1的剂量灌服,4 h后三七总皂苷各组灌服不同剂量三七总皂苷溶液(100、200、400 mg.kg-1.d-1),模型组灌服等体积生理盐水。分别于实验第12、16、20周测定大鼠肾组织SOD活性、MDA含量及GHS含量。结果三七总皂苷可升高慢性马兜铃酸肾病大鼠肾组织SOD活性和GSH-PX含量,明显降低肾组织中MDA(P<0.05)。结论三七总皂苷对慢性马兜铃酸肾病大鼠损伤起保护作用。

Objective To observe the antioxidation of panax notoginseng saponins（PNS） in rats of chronic aristolochic acid nephropathy（ANN）.Methods Wistar rats were randomly divided into control group,model group and panax notoginseng saponins groups（low,medium and high dose）.The model group and PNS group were given the extract of Caulis oristolochiae Manshuriensis（according to the dose of aristolochic acid 20 mg·kg-1·d-1）by intragastric administer for one week.And then the doses were changed of 15 mg·kg-1·d-1.Four hours later,the PNS group was subdivided into three groups and administered PNS（100,200,400 mg·kg-1·d-1）respectively.The model group was given equal volume of normal sodium.The urine and blood were collected at the end of the 12th,16th,20th week.Changes of the activity of superoxide dismutase（SOD） and the content of malondialdehyde（MDA） and Glutathione peroxidase（GSH-PX） in kidney were observed.Results PNS could enhance activity of SOD and GSH-PX,reduce the content of MDA in kidney（P0.05）.Conclusion PNS had protective effects on rats of chronic aristolochic acid nephropathy.