摘要
建立了采用超高效液相色谱同时测定饮料中安赛蜜、苯甲酸、山梨酸和糖精钠的方法。样品经简单预处理后,使用ZORBAX ECLIPSE XDB-C18色谱柱分离,以甲醇和乙酸铵溶液(0.02 mol/L)为流动相进行等度洗脱,流速为2 mL/min,二极管阵列检测器检测,波长230 nm,柱温:30℃,进样分析时间仅需2 min。4种添加剂在0.5~40.0μg/mL范围内呈良好线性关系,相关系数r〉0.999;精密度(RSD,n=6)〈3.0%;在加标水平为5,20和40μg/L时,被测物的平均回收率为88.3~98.2%。说明该方法简便、快速、准确、回收率高,适用于大批饮料样品上述4种食品添加剂的同时检测。
An ultra performance liquid chromatographic(UPLC) method for the simultaneous determination of acesulfame,benzoicacid,sorbicacid and sodium saccharin in beverage was developed.The samples were separated on an ZORBAX ECLIPSE XDB-C18 column by isocratic elution at 230nm.With the four target compounds working curve in the scope of 0.5~40.0 μg/mL,a good liner relationship was found between the concentration and peak area(r0.999) with precision(RSD,n=6)3.0%.The average recoveries in samples were 88.3~98.2%.The method is sinple,rapid and accurate with high recovery rate.So it can be used to detect these four kinds of food additives in beverage.
出处
《现代食品科技》
EI
CAS
2011年第5期601-602,533,共3页
Modern Food Science and Technology
关键词
超高效液相色谱
饮料
安赛蜜
苯甲酸
山梨酸
糖精钠
ultra performance liquid chromatography(UPLC)
beverage
acesulfame
benzoicacid
sorbicacid
sodium saccharin