超高效液相色谱法快速测定饮料中的安赛蜜、苯甲酸、山梨酸和糖精钠

Rapid Determination of Acesulfame,Benzoicacid,Sorbicacid and Sodium Saccharin in Beverage by Ultra Performance Liquid Chromatography

建立了采用超高效液相色谱同时测定饮料中安赛蜜、苯甲酸、山梨酸和糖精钠的方法。样品经简单预处理后,使用ZORBAX ECLIPSE XDB-C18色谱柱分离,以甲醇和乙酸铵溶液（0.02 mol/L）为流动相进行等度洗脱,流速为2 mL/min,二极管阵列检测器检测,波长230 nm,柱温：30℃,进样分析时间仅需2 min。4种添加剂在0.5~40.0μg/mL范围内呈良好线性关系,相关系数r〉0.999;精密度（RSD,n=6）〈3.0%;在加标水平为5,20和40μg/L时,被测物的平均回收率为88.3~98.2%。说明该方法简便、快速、准确、回收率高,适用于大批饮料样品上述4种食品添加剂的同时检测。

An ultra performance liquid chromatographic（UPLC） method for the simultaneous determination of acesulfame,benzoicacid,sorbicacid and sodium saccharin in beverage was developed.The samples were separated on an ZORBAX ECLIPSE XDB-C18 column by isocratic elution at 230nm.With the four target compounds working curve in the scope of 0.5~40.0 μg/mL,a good liner relationship was found between the concentration and peak area（r0.999） with precision（RSD,n=6）3.0%.The average recoveries in samples were 88.3~98.2%.The method is sinple,rapid and accurate with high recovery rate.So it can be used to detect these four kinds of food additives in beverage.