摘要
应用RP－HPLC方法检测血清中氟脲嘧啶（Fluorouracil）浓度，本方法采用C18分析柱及预柱，流动相为甲醇-0．5％乙酸水溶液（5：95v／v），检测波长266nm，血清样品用乙酸乙酯萃取，氮气吹干，血药浓度在25ng～40μg／ml范围内呈线性关系，相关系数0．9999，最低检测浓度为25ng／ml。生物样品的方法回收率为101．2％～107．7％，日内日间变异系数在1．13％～6．24％（n＝5）。
A method was developed for fluorouracil concertration in human serum by RP-HPLC. The analytical and preparing column was filled with a c18 stationary phase,the mobile phase consisted of a mix-ture of methanol-0. 5% acetic acid solution (5: 95v/v). The detective wavelength was 266nm. The fluo-rouracil in human serum can be extracted well with ethyl acetate and be blowed dry with N2. The calibra-tion curve was linear in the range from 0.025 to 40 μg/ml (r = O. 9999), the limit of determination was 25ng/ml;the rate of recovery 101. 2 % to 107. 7 %. the RSD within day and between day were lower than 6.24% (n = 5).
出处
《海峡药学》
1999年第3期35-36,共2页
Strait Pharmaceutical Journal
