HCV感染小鼠小胶质细胞后TLR7mRNA和IL-6表达

Expressions of TLR7 mRNA and IL-6 in mouse microglial cells after hepatitis C virus infection

目的通过观察丙型肝炎病毒(HCV)感染小鼠小胶质细胞BV2后Toll样受体(TLR)7 mRNA及白细胞介素(IL)-6水平的动态变化,探讨HCV对中枢神经系统(CNS)的免疫发病机制。方法用20%(体积分数)的HCV阳性血清感染BV2细胞,在感染后6、12、244、8 h收集细胞,应用实时荧光定量PCR(RT-qPCR)和ELISA法检测TLR7 mRNA和IL-6表达,同时设空白对照组和正常血清组。结果 3组BV2细胞中均有TLR7 mRNA表达;HCV阳性血清感染BV2细胞后TLR7 mRNA表达随时间延长而逐渐增高,其中在12、24、48 h时表达水平均较其他两组增高(P<0.05)。IL-6表达在HCV阳性血清组也逐渐增高,48 h时与空白对照组、正常血清组48 h时比较差异有统计学意义(P<0.05)。结论 BV2细胞可以表达TLR7 mRNA,HCV感染能上调BV2细胞TLR7 mRNA和IL-6表达。TLR7可能参与了HCV对CNS的病理生理过程。

Objective To observe the dynamic changes of TLR7 gene in mouse microglial BV2 after hepatitis C virus （HCV） infection and to explore the immunological pathogenesis of HCV. Methods BV2 cells were infected with the positive serum containing 20% HCV. Cells and supernatants were collected at 6 h, 12 h, 24 h and 48 h after infection, then TLR7 mRNA and IL-6 were detected by real time quantitative PCR （RT qPCR） and enzyme-linked immunosorbent assay （ELISA）, respectively. Two control groups were included, one was without any treatment and another was treated with serum. Results TLR7 mRNA expressed in all three groups. TLR7 mRNA in the HCV-infected group increased gradually along with the time, and the increase was significant at 12 h, 24 h and 48 h in comparison with the other two control groups （P〈0.05）. IL-6 in the HCV- infected group increased gradually, also, and was significantly higher at 48 h compared with other two groups （P〈0.05）. Conclusions BV2 cells can express TLR7 mRNA, HCV infection can upregulate the expressions of TLR7 mRNA and IL-6 in BV2 cells. TLR7 might be involved in the HCV pathophysiology in central nervous system.