天花粉蛋白、米非司酮和顺铂对卵巢癌细胞HLA-G表达的影响

The effects of trichosanthin,mifepristone,cisplatin on the expression of HLA-G in ovarian cancer cells

目的检测HLA-G在卵巢癌细胞中表达情况,探讨天花粉蛋白、米非司酮、顺铂是否能够通过下调HLA-G的表达,达到抑制肿瘤生长的目的。方法采用RT-PCR技术检测卵巢癌卵巢浆液性囊腺癌(SKOV3)、卵巢粘液性囊腺癌(3AO)、卵巢腺癌细胞株(OVCAR3)细胞中HLA-G表达情况,选择出表达HLA-G阳性的细胞株。MTT技术观察不同浓度天花粉蛋白、米非司酮、顺铂对该细胞体外生长增殖能力的影响及对细胞中HLA-GmRNA表达水平的影响。结果卵巢癌OVCAR3细胞株表达HLA-G为阳性,SKOV3和3AO细胞表达均为阴性;天花粉蛋白、米非司酮、顺铂均能抑制OVCAR3细胞生长,并呈浓度依赖性;天花粉蛋白、米非司酮均能明显下调OVCAR3细胞中HLA-GmRNA表达水平(P<0.05),而顺铂对OVCAR3细胞中HLA-GmRNA表达无明显影响(P>0.05)。结论天花粉蛋白、米非司酮和顺铂都具有抑制卵巢癌细胞生长的作用,前二者治疗卵巢癌的作用机制同顺铂有所不同,与顺铂联合应用,可能会取得更好的治疗效果。

Objective To observe the expression of HLA-G in ovarian cancer cells and to explore whether the expression of HLA-G can be down-regulated by trichosanthin,mifepristone and cisplatin,resulting in the inhibition effect on tumor growth.Methods RT-PCR was used to detect the expression of HLA-G in human ovarian cancer SKOV3,3AO,OVCAR3 cells and the HLA-G mRNA levels after these cells were treated with trichosanthin,mifepristone,cisplatin respectively.The MTT method was performed to observe the influence of the three drugs on the proliferation of ovarian cancer cells.Results The expression of HLA-G mRNA was positive only in OVCAR3 cells.The trichosanthin,mifepristone,cisplatin inhibited the proliferation of ovarian cancer cells obviously,and the inhibition rate was dependent on the drug concentration.Trichosanthin and mifepristone could down-regulate HLA-G mRNA levels significantly,but the difference for cisplatin was not significant.Conclusion Trichosanthin,mifepristone,cisplatin can inhibit the growth of ovarian cancer cells obviously.The anti-tumor action mechanism of trichosanthin and mifepristone is different from that of cisplatin,and the combination application of cisplatin and trichosanthin or mifepristone can obtain better therapeutic effect.