厄贝沙坦干预对DN大鼠炎性细胞因子的影响

Influence of irbesartan intervention on inflammatory cytokines in rats with diabetic nephropathy

目的观察厄贝沙坦干预对炎性细胞因子白介素-6(IL-6)、IL-8、IL-10和C-反应蛋白(CRP)水平的影响,并从炎症方面探讨其可能的药物作用机制。方法将36只Wistar大鼠随机分为右肾切除对照组(C组)、糖尿病肾病组(DN组)、厄贝沙坦治疗组(Irb组)3组,每组12只。第8、12周末收集大鼠血液标本,ELASA方法测定血液IL-6、IL-8、IL-10和CRP含量。结果 Irb组和DN组8周、12周的CRP、IL-6、IL-8均高于C组;而DN组8周、12周的CRP、IL-6、IL-8又高于Irb组(P<0.01)。Irb组和DN组8周、12周的IL-10均低于C组,而DN组8周、12周的IL-10又低于Irb组(P<0.01);C组12周与8周的上述各指标无明显变化。结论厄贝沙坦不同程度地抑制DN大鼠IL-6、IL8和CRP的产生,刺激IL-10的分泌,从而部分逆转炎性细胞因子和抗炎细胞因子的失衡,降低蛋白尿、抑制肾小球的硬化,而延缓糖尿病肾病的进展。

Objective To observe the effect of irbesartan intervention on the levels of inflammatory cytokines including interleukin-6（IL-6）,interleukin-8（IL-8）,interleukin-10（IL-10） and C-reactive protein（CRP） in rats with diabetic nephropathy（DN）,and to investigate the possible drug action mechanism.Methods 36 Wistar rats were randomly divided into three groups：control group（right nephrectomized）,diabetic nephropathy group（DN group） and irbesartan treatment group,and there were 12 rats in each group.At the end of the 8th and the 12th week,the serums of the rats were collected.The levels of IL-6,IL-8,IL-10 and C-reactive protein in serum were detected by ELASA.Results At the end of the 8th and the 12th week,the levels of IL-6,IL-8 and CRP in irbesartan treatment group and DN group were significantly higher than those in control group,and the levels of IL-6,IL-8 and CRP in DN group were significantly higher than those in irbesartan treatment group（P0.01）.At the end of the 8th and the 12th week,the levels of IL-10 in irbesartan treatment group and DN group were significantly lower than those in control group,and the levels of IL-10 in diabetic nephropathy group were significantly lower than those in irbesartan treatment group（P0.01）.Conclusion Irbesartan can inhibit the production of IL-6,IL-8 and CRP and can stimulate the secretion of IL-10,as a result,which can improve partly the unbalance of inflammatory cytokines and anti-inflammatory cytokines,decrease and inhibit glomerular sclerosis,thereby,which can delay the advancement of diabetic nephropathy.