局部应用促红细胞生成素对周围神经再生的实验研究

Experimental study of the effect of local application of recombinant human erythropoietin on peripheral nerve regeneration in rats

目的探讨局部应用人重组促红细胞生成素(recombinant human ythropoietin,rh-EPO)对大鼠坐骨神经断裂后神经再生的作用。方法选用健康雄性Wistar大鼠16只,显露其左侧坐骨神经,于梨状肌出口1.0 cm处切除坐骨神经5 mm,两端用硅胶管桥接形成神经再生室。实验动物随机分为2组,每组8只,EPO组:再生室内注入重组人促红细胞生成素5 000 U/kg;对照组:注入同体积的0.9%氯化钠溶液。术后第8周分别进行坐骨神经功能指数(SFI)、电生理检测、小腿三头肌湿重测定。结果术后第8周2组SFI、运动神经潜伏期延迟比、运动神经波幅恢复比及小腿三头肌湿重恢复比测定结果差异均有统计学意义(P<0.05)。结论局部应用rh-EPO能促进周围神经再生和功能恢复。

Objective To investigate the effect of local application of recombinant human erythropoietin（rh-EPO） on peripheral nerve regeneration in rats.Methods 16 healthy male Wistar rats were enrolled in the stydy.The rats＇ left sciatic nerve was exposed,then 5mm sciatic nerve segment was removed in 1.0cm position from the piriform muscle export.The regeneration chambers were made by using silicone tube to connect the proximal and distal ends.The experimental rats were randomly divided into two groups： EPO group and control group,8 rats in each group.The rh-EPO 5 000U/kg was locally injected to the regeneration chamber in EPO group,while normal saline solution was used in control group.On the 8th week after operation,the sciatic nerve function index（SFI）,electrophysiological parameters and the wet weight of triceps surae muscle were detected.Results On the 8th week after operation,these parameters in EPO group were superior to those in control group（P0.05）.Conclusion The local application of rh-EPO can promote the regeneration of injured peripheral nerve and can improve the recovery of the nerve function.