MMP-7和PTEN在非小细胞肺癌的表达及其意义

Expression of MMP-7 and PTEN in non-small cell lung cancer and its significance

目的:研究MMP-7和PTEN在非小细胞肺癌(NSCLC)组织中及癌旁正常肺组织的表达程度,探讨两者表达水平与肺癌侵袭、转移及相关临床病理特征之间的关系,为NSCLC的发生机制提供理论基础,并且为其早期诊断提供新的思路。方法:用免疫组织化学的方法检测60例NSCLC标本及20例癌旁正常肺组织中MMP-7和PTEN蛋白的表达水平。结果:NSCLC组织中MMP-7的表达率为80%,显著高于癌旁肺组织阳性率20%,两者比较差异有统计学意义(P<0.01),MMP-7蛋白的表达与NSCLC的临床分期、分化程度及淋巴结转移密切相关(P<0.05);PTEN在NSCLC组织中的阳性率为55%,显著低于癌旁正常肺组织的阳性率90%,两者比较差异有统计学意义(P<0.01),PTEN蛋白的表达与肺癌组织的临床分期、细胞分化程度和淋巴结转移密切相关(P<0.05);两者均与其它的临床病理因素无关(P>0.05),MMP-7与PTEN两者的表达呈负相关(r=-0.376,P<0.05)。结论:联合检测MMP-7与PTEN蛋白的表达对预测NSCLC患者的浸润、转移和预后均有重要意义。

Objective：To study the correlation between the expression of MMP-7 and PTEN with clinical pathological characteristic invasion and metastasis in an attempt to investigate their expression levels in NSCLCs and its corresponding adjacent tissue and provide the theoretical foundation for the mechanism of NSCLC and new thinking for its early diagnosis.Methods：The expression levels of MMP-7 and PTEN in 60 cases of NSCLCs and 20 cases of corresponding adjacent tissue were detected by SP immunohistochemistry.Results：The positive rate of MMP7（80%） in lung cancer was significantly higher than that of normal lung tissue（20%）,with a significant difference（P0.01）,the expression of MMP-7 had close relations to tumor clinical staging,differentiation degree and lymph node metastasis（P0.05） and the positive rate of PTEN（55%） in NSCLCs was significantly lower than that of normal lung cancer by organization（90%）,with a significant difference（P0.01）.The expression of PTEN had close relations to tumor clinical staging,differentiation degree,and lymph node metastasis（P0.05）,both having no relation to other clinical pathological factors（P0.05）,and the MMP-7 expression had a significant negative correlation with the PTEN expression（r=-0.376,P0.05）.Conclusion：The combined detection of the two proteins may be useful in predicting the development and prognosis of NSCLC.