从传代细胞株中去除支原体污染的研究

STUDIES ON ELIMINATION OF MYCOPLASMAS FROM CELL LINE CULTURES

用抗菌药物从传代细胞株中去除支原体污染的试验结果表明,卡那霉素和庆大霉素对支原体均无明显的杀灭作用。用10μg/ml 的 Tiamutin 处理支原体的效果较好。采用单克隆细胞稀释、选择法与 Tiamutin(10μg/ml)处理相结合,经电镜观察可去除细胞中支原体的污染。检查支原体的方法是否特异、敏感、快速是对试验结果正确判断的一个重要的关键。为了防止支原体的污染,除了加强对原材料(包括小牛血清、培养液等)的检查以及把住严格的无菌操作条件外,必要时可在培养基中加入10μg/ml 的 Tiamutin。

Mycoplasmas were effectively eliminatedfrom contaminated LTK and CHO cell linesby means of the antibiotic Tiamutin(10ug/ml),while Kanamycin and Gentamycin wereless potential.An elimination procedurewhich involved the consecutive treatment ofthe cell cloning over a periods of 21—28days was adopted.This procedure was ef-fective when applied to cell lines contaminat-ed with unidentified and partially non-cul-tivable strains of mycoplasmas which could bedetected by electronic microscope.