COMT基因多态性与子宫内膜异位症和子宫腺肌病关系研究

Study on the Correlation Between Gene Polymorphism of COMT and Endometriosis and Adenomyosis

用蛋白酶K消化-饱和酚氯仿法提取外周血白细胞基因组DNA,运用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法分别对61例内异症(EMs组)、59例子宫腺肌病(Ad组)和65例非内异症及腺肌病女性(对照组)进行基因分型,以探讨COMT基因多态性与子宫内膜异位症和子宫腺肌病的关系.结果显示:1)内异症组、腺肌病组和对照组G/G,G/A,A/A基因型频率和A等位基因频率差异均无统计学意义;2)G/G+G/A基因型患内异症和腺肌病的风险分别为G/G基因型的0.629倍和0.876倍,差异无统计学意义.由此得出,COMT基因NlaⅢ多态性与子宫内膜异位症和子宫腺肌病无相关性,子宫内膜异位症和子宫腺肌病发病机理具有相似性.

To study the correlation between gene polymorphism of Catechol-O-Methyl transferase（COMT） and endometriosis（EMs） and adenomyosis（Ad）,the polymorphism of COMT gene was analyzed by polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP） method in 61 patients with EMs and 59 patients with Ad and 65 healthy controls.There was no significance of the alleles and genotype between EMs group,Ad group and control group.The relative risk suffered from endometriosis of G/A and A/A genotype was 0.629 and 0.876 times of the G/G genotype respectively.There was no correlation between the NlaⅢ gene polymorphism of COMT and endometriosis and adenomyosis.The mechanism of endometriosis and adenomyosis was similar.