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三种HIV抗体确证试剂盒检测早期感染的比较 被引量:15

Comparison of three HIV antibody confirmatory assay kits in confirming early HIV infection
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摘要 目的 比较3种HIV抗体确证试剂盒检测HIV早期感染的性能.方法 对5份HIV抗体阳性血浆样品进行10倍系列稀释,然后用ELISA检测.对检测结果呈阳性反应的稀释样品分别用3种HIV抗体确证试剂盒进行检测以测试其灵敏度,所用试剂盒包括北京万泰公司的HIV 1+2型抗体检测试剂盒(万泰RIBA)、新加坡MP公司的HIV 1+2型抗体检测试剂盒(MP-WB)和比利时Innogenetics公司的INNO-LIA TM HIV Ⅰ/ⅡScore(INNO-LIA).用这些试剂盒检测11套HIV抗体阳转血清盘中ELISA试验呈阳性反应的样品(共48份).结果 对HIV抗体阳性稀释样品的检测结果显示,当5份样品在稀释100倍时,万泰RIBA均检测出阳性结果.在ELISA试验呈阳性反应的48份HIV抗体阳转血清盘样品中,万泰RIBA、MP-WB和INNO-LIA的确证阳性率分别为97.92%(47/48)、81.25%(39/48)和91.67%(44/48).万泰RIBA与MP-WB之间差异有统计学意义(χ2=6.13,P<0.05),INNO-LIA与MP-WB之间差异有统计学意义(χ2=5.48,P<0.05),而万泰RIBA与INNO-LIA之间差异无统计学意义(χ2=1.33,P>0.05).对于含有HIV抗体检测结果不确定样品的6套阳转血清盘,用万泰RIBA、MP-WB和INNO-LIA检测的平均阳转时间分别为0.7、13.3、3.7 d.结论 与我国目前常用的MP-WB相比,万泰RIBA和INNO-LIA可以缩短HIV抗体确证的窗口期. Objective This study was to compare the performance of three HIV antibody confirmatory assay kits in confirming early HIV infection. Methods Five HIV antibody-positive plasma specimens were ten-fold serially diluted and then detected by ELISA. The above diluted specimens were detected with the following three HIV antibody coufirmatory assay kits to analyze their sensitivity, including Wantai-RIBA ( Recombinant immunoblot assay, Beijing Wantai Biological Pharmacy, China), MP-WB ( HIV Blot 2. 2 WB,MP Biomedicals Asia Pacific Pte. Ltd. ,Singapore) and INNO-LIA ( INNO-LIATM HIV Ⅰ/Ⅱ Score, Innogenetics N. V. , Belgium), respectively. These kits were further used to detect 48 ELISA-reactive specimens from 11 sets of HIV seroconversion specimens (a total of 48 sanples ) which were previously detected as HIV antibody-positive by ELISA. Results When 5 samples were diluted to 100 fold,Wantai-RIBA still can detect them positive. Among the 48 HIV antibody-positive specimens detected with ELISA,the confirmation positive rate for Wantai-RIBA, MP-WB and INNO-LIA were 97.92% (47/48),81.25 % ( 39/48 ) and 91.67% ( 44/48 ), respectively. There was statistically significant difference between the confirmatory results of Wantai-RIBA and MP-WB ( χ2 = 6. 13, P 〈 0. 05 ), as well as between those of INNO-LIA and MP-WB ( χ2 = 5.48, P 〈 0. 05 ); however, there was no statistically significant difference between those of Wantai-RIBA and INNO-LIA ( χ2 = 1.33, P 〉 0. 05 ). For other six HIV seroconversion panels containing indeterminate specimens, the average seroconversion period of time for Wantai-RIBA,MP-WB and INNO-LIA were 0. 7,13.3 and 3.7 days, respectively. Conclusion Compared with MP-WB,Wantai-RIBA and INNO-LIA could reduce the window period to confirm early HIV infection.
出处 《中华预防医学杂志》 CAS CSCD 北大核心 2011年第5期430-434,共5页 Chinese Journal of Preventive Medicine
基金 基金项目:国家“十一五”重大科技专项(2008ZX10001-013)
关键词 HIV 抗体 艾滋病血清学诊断 确证试验 HIV Antibodies AIDS serodiagnosis Confirmatory assay
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  • 1中国疾病预防控制中心.HIV抗体检测.《全国艾滋病检测技术规范》(2004年修订版).2004年9月.
  • 2Niel Conatantine.HIV Antibody Assays,HIV inSite Knowledge Base Chapter,May 2006.http://hivinsite,ucsf.edu.
  • 3Cremonezi D,Mesquita PE,Romao MM,et al.Prevalence of indeterminate human immunodeficiency virus western blot results in pregnant women attended at a public hospital in Presidente Prudente,Brazil.Braz J Infect Dis,2005,9(6):506-509.
  • 4Celum CL,Coombs RW,Lafferty W,at al.Indeterminate human immunodeficiency virus type Ⅰ western blots:seroconversion risk,specificity of supplemental tests,and an algorithm for evaluation.J Infect Dis,1991,164(4):656-664.
  • 5Weber B, Fall EH, Berger A, et al. Reduction of diagnostic window by new fourth-generation human immunodeficiency virus screening assays. J Clin Microbiol, 1998,36:2235-2239.
  • 6Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004,122 : 185 - 194.
  • 7Ly TD, Edlinger C, Vabret A. Contribution of combined detection assays of p24 antigen and anti-human immunodeficiency virus (HIV) antibodies in diagnosis of primary HIV infection by routine testing. J Clin Microbiol, 2000, 38 : 2459-2461.
  • 8Polywka S, Duttmann H, Lubben F, et al. A new combined HIV 1,24 antigen and anti-HIV-1/2/O screening assay. Methods Mol Biol, 2005,304 : 229 -243.
  • 9Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods,1998,75:27-38.
  • 10Weber B, Meier T, Enders G, et al. Fourth generation human immunodeficiency virus (HIV) screening assays with an improved sensitivity for p24 antigen close the second diagnostic window in primary HIV infection, J Clin Virol, 2002, 25:357-359.

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