PCR检测HPV DNA诊断女性生殖道尖锐湿疣

APPLICATION OF PCR TECHNIQUE TO DETECT HPV DNA IN THE DIAGNOSIS OF ACUMINATE WART IN FEMALE GENERATIVE TRACT

目的：应用聚合酶链反应（RCR）检测HPVDNA诊断女性生殖道尖锐湿疣。方法：应用PCR法对门诊女性生殖道尖锐湿疣标本中HPV6．11型进行检测。结果：9例病理学检测阳性标本有8例检测出HPV6.11望病毒．20例病理检测阴性标本也有4例检出HPV6．11型病毒。结论：PCR是诊断尖锐湿疣快速、简便、敏感性高和特异性强的方法。病因不明的假性湿疣的一部分病例可能是尚未出现典型病变的早期尖锐湿疣。

Objective: Apply PCR technique to detect HPV DNA to diagnose acuminate wart in female generative tract. Methods: Apply PCR technique to detect HPV6. 11 type in acuminate wart,samples from the generative tract of 29 outpatients. Results: Nine samples are positive pathologically, of which eight samples are detected to have been infected with HPV6. 11 type, four samples out of twenty which are negative pathologically are detected to have been infected with HPV6.11 type. Conclusion: PCR is a fast,convenient technique with high sensitivity and specificity to diagnose acuminate wart. some pseudowart samples with unknown pathology might be the early stage of acuminate wart.