bFGF对大鼠骨髓细胞骨形成的影响

The Effect of Basic Fibroblast Growth Factor to the Formation of the Marrow Cell of Rat

为了探讨碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对骨髓细胞系的作用 ,采用H3_TdR法、比色法和VonKossa染色法,检测bFGF作用下骨髓细胞增殖、分化、骨形成情况。结果持续使用bFGF时 ,其促进骨髓细胞增殖 ,并向成骨细胞分化。阶段性使用bFGF时 ,骨形成分化初期 ,bFGF促进骨髓细胞增殖 ,分化开始后 ,bFGF抑制成骨细胞成熟。

Objective: To study the effect of bFGF on marrow line cells, we determined the marrow cells' proliferation、differentiation、bone formation states by the effect of bFGF using H3-TdR colorimetry and Von Kossa staining. Results: Continue to add bFGF, it can promote the proliferation of marrow cells and promote them differentiate to osteoblast. The stage added bFGF, bFGF can promote the marrow cells proliferate at the primary of bone formation differentiation. But after differentiation, bFGF will restrain osteoblast mature.