噻庚啶、山莨菪碱对内毒素休克TNF_α表达的影响

Effects of cyproheptadine and anisodamine on TNF_α gene expression in endotoxic shock

目的：研究噻庚啶山菪碱对内毒素休克TNFα况表达的影响。方法：Wistar臣鼠脉注射脂多糖（LPS，EcoliO111B45mg/kg)复制内毒素休克模型。Northem印迹杂交分析肝脏TNFαmRNA表达，放射免疫法测定血浆TNFαW的含量。结果：LPS攻击后2h，大鼠肝脏TNFαmRNA表达显著高于盐水对照；（杂交信号经扫描仪半定量分析对．兀19幼。盐水对照11．防止7．m，P＜001）；血浆TNFα流水平明显升高：（211.52±3.16）μg/LvsLPS攻击前（2.19±0．95）μg/L，P＜0．01。静脉注射IPs后立即给予噻庚啶（Cyp，5mg/kg）、山茛菪碱（Ami，10mg/kg），均能显著抑制LPS诱导的大鼠肝脏TNFαmRNA表达，降低血浆TNFα流含量；提高休克大鼠的平均动脉血压（MAB）及休克小鼠的24h的存活率。结论：Cyp和Ani均显著抑制内毒素休克TNFα基因表达，具有较强的抗休克作用。

To study the effects of cypldheptadine (Crp) and whwhainine (Mi ) on TNF, gene expression in endo-totic shock. METH0DS: Endototic shock in rat5 was pnxluced by iv injection of LPS (E c0li 0lllB4, 5 twkg). TNFa mR-NA leveis in rat liver was assessed by NoIthem blot. Plasma TNFa conents were detendned by radiobounoassay. RE-SULTh:The wi mRN levels in rat liver at 2 h aller us challenge was increased ohviously (autoIadipo were ana-mp by scedng: 37 .76 l 9. 84 vs saline contol 11. 26 t 7. 60, P < 0. Ol ); The plasma TNF contents wns increased^ehly before ffe challeng (2l -52 l 3. l6)ud/L vs (2- l9 t O. 95)ug/L, P < 0.0l. C)P 5 mg;kg or Ani 1O ndkg^diately injected after iv us inaikde decreased the Wi inRNA levels in liver and plasma TNFo contents at 2 h alleriv bo, and eleVated the mean arterial blnd pressare (MABP) 0f the shocked un. Crp and Mi rnarediy boroved il1ew s^al rate 24 h after ffe(20 ndkg) challenge. C0NCLUSI0N: TNF, gene exPression increased 0bviousl} in en-do0tic shock. CyP and Mi strongly whiliited usin(luced TNF gene expression, and have a beneficial antishock effects.