单侧输尿管梗阻后小鼠肾脏环氧化酶-2的表达及其意义

Expression of cyclooxygenase-2 in kidneys of mice with unilateral ureteral obstruction

目的：观察环氧化酶-2（COX－2）在单侧输尿管梗阻小鼠梗阻肾脏内的表达情况，探讨COX-2在炎性肾脏疾病中清理生理作用。方法：利用阿-PCR检测了COX－2在小鼠单侧输尿管梗阻后4h、24h、72h、sd和7d的表达变化并采用PAS染色观察上述各时间点的肾组织病理改变。结果：单侧输尿管梗阻术后4h肾皮质中的COX－2的表达开始增高，72h达到高峰。术后第一天肾间质中可见少量炎细胞浸润，第3d明显增多，第7d达到高峰。结论：本研究提示COX－2的过度表达很可能是单侧输尿管便用后肾内炎细胞浸润和积聚的起站或促进因素之一。

Cyclooxygenase-2(COX- 2) is an inducible cyclooxygenase which may Play an important role in in-flammatory diseases.METHODS: To examine the involvement of COX-2 in the inflammatory renal diseases in vivo the expression of this was detected in kidneys of mice that receiving unilateral ureteral obstruction(UUO) at 4-hours, 24-hours, 72 - hours, 5-day, 7-dsys by RT - PCR methed. The morphological changes of the obstructed kidneys wereobserved at the same time point. RESULTS:The regults demonstrated that the expression of COX-2 mRNA were beginningto imcrease at 4-hours , were up to peak at 72 -hare after UUO operation. A few numbers of infiltrating inflammatory cellswere found at 24 - hours, a large humbers of infiltration inflammatory cells were found 72 -hours, and the peak of the num-bers of those cells was at 7-days after UUO operation. CONCLUSIO: COX-2 expression is upreghated in the obstract-ed kidneys of UUO mice.The overexpression of COX-2 may be an inducing or promoting factor in inflammatory cells infil-tration in the kidney of UUO mice.