血管紧张素转化酶基因多态性与子痫前期

Polymorphisms of angiotensin-converting enzyme gene in pre-eclampsia

目的探讨孕妇和新生儿血管紧张素转化酶(ACE)基因变异是否与子痫前期有关联。方法应用多聚酶链反应-限制性片段长度多态性分析法(PCR-RFLP),对四川地区43例汉族子痫前期患者和44例健康对照孕妇及其两组新生儿血管紧张素转化酶基因多态性进行检测;同时用紫外分光光度法检测孕妇和新生儿脐血血管紧张素转化酶活性水平。结果子痫前期组和正常孕妇组ACE基因D等位基因频率两组比较无统计学差异(37.2%vs 29.5%,P>0.05);早发型子痫前期组和晚发型子痫前期组ACE基因D等位基因频率比较无统计学差异(32.4%vs 40.4%,P>0.05);子痫前期新生儿组和正常新生儿组ACE基因D等位基因频率比较无统计学差异(47.1%vs 32.5%,P>0.05)。子痫前期组DD基因型携带者较II基因型携带者ACE活性水平显著增高(44.01±12.41 vs 29.96±11.26,P<0.05)。结论本研究未发现孕妇及新生儿ACE基因变异与子痫前期有关,但子痫前期组DD基因型携带者ACE活性水平显著增高。

Objective To observe the relationship between the genotype and allele frequency of angiotensin-converting enzyme（ACE） gene in mothers and fetus and pre-eclampsia.Methods Using the technique of polymerase chain reaction and restriction fragment length polymorphism,the relationship of the polymorphisms of ACE was analyzed between in 43 pregnant women with preeclampsia and 44 healthy pregnant women,including their fetus.Results No significant differences in allele and genotype frequencies of ACE gene were found between patients with preeclampsia and the normal control subjects.Allele D frequencies were 37.2% and 29.5%（P0.05） respectively.The frequency of the D allele in patients with early onset pre-eclampsia was significant higher than that in patients with late onset pre-eclampsia and allele C frequencies were 32.4% and 40.4%（P0.05）respectively.The frequency of the D allele in fetus whose mothers with pre-eclampsia was significant higher than that in fetus of the normal control subjects and allele C frequencies were 47.1% and 32.5%（P0.05）.Conclusion There is relation between ACE gene allele mutation in mothers and early onset pre-eclampsia.There is relation between ACE gene allele mutation in fetus and pre-eclampsia too.