青蒿素联合^(60)Co γ射线对HeLa和SiHa细胞所致DNA损伤的研究

Study on Effect of Artemisinin Combined with ^(60)Co γ-ray on DNA Damage in HeLa and SiHa cells

目的探讨青蒿素联合^(60)Coγ射线照射后对人宫颈癌HeLa和SiHa细胞的DNA损伤。方法采用甲基四唑蓝(MTT)法检测不同浓度青蒿素对人宫颈癌HeLa和SiHa细胞的抑制效应,确定青蒿素的最适实验浓度;采用单细胞凝胶电泳技术(SCGE)检测照射后HeLa和SiHa细胞DNA的损伤情况。结果青蒿素对HeLa和SiHa细胞的抑制率存在剂量-效应关系;SCGE结果显示:两种细胞的单药组与对照组的彗星细胞检测指标(拖尾率、尾长、OLIVE尾矩和彗尾DNA%)无明显差异(P>0.05);在相同照射剂量下,青蒿素加照射组的HeLa细胞的彗星细胞检测指标高于单纯照射组(P<0.05),而SiHa细胞差异无统计学意义(P>0.05)。结论青蒿素本身不能引起He-La和SiHa细胞DNA损伤,但辐照后可致HeLa细胞DNA损伤加重,增加了HeLa细胞的辐射敏感性,而对SiHa细胞没有辐射增敏作用。

Objective To investigate the effect of Artemisinin combined with ^60Coγ-ray on DNA damage in HeLa and SiHa cells of human cervical cancer.Methods Cell growth kinetics was evaluated by MTT assay to determine the most appropriate drug concentration.Effects of Artemisinin combined with^60Coγ-ray on DNA damage in HeLa and SiHa cells were detected by single cell gel electrophoresis. Results With the concentration increased during the effect of Artemisinin,the HeLa and SiHa cells had higher inhibition on cell proliferation.The SCGE showed that：the comet cell analysis indexes（the comet cells ratio、Tail Length、Olive Tail Moment and Tail DNA%） there was no statistic difference in between the artemisinin group and the control group（P〈0.05）.With radiation in the same dose,the comet cell analysis indexes of Hela cells treated with both artermisinin and exposed to radiation were higher than that only exposed to radiation group（P〉0.05）,but there was no difference in SiHa cells（P〉0.05 ）.Conclusion Artemisinin can not induce DNA damage in both HeLa and SiHa cells,but it can make irradiated HeLa cells DNA damage to be aggravated and enhance HeLa cells＇ radiation sensitivity.However,Artemisinin has no radiosensitizing effect on SiHa cells.