环孢素A壳聚糖纳米微粒抑制兔结膜成纤维细胞增生的作用

Inhibitive Effects on Conjunctival Fibroblasts Proliferation in Rabbits of Cyclosporin A-loaded Chitosan Nanoparticles

目的研究自制的环孢素A壳聚糖纳米微粒[CS(CsA)-NP]对兔结膜成纤维细胞(RCFs)增生的抑制作用。方法取第4～6代培养的兔结膜成纤维细胞,分别加入CS(CsA)-NP、环孢素A(CsA)原药、壳聚糖纳米微粒(CS-NP)及平衡盐溶液,用四甲基偶氮唑盐(MTT)比色法检测对RCFs的抑制作用。结果 CS(CsA)-NP、CsA原药、CS-NP以质量浓度和时间依赖方式抑制RCFs的增生。对RCFs的抑制作用CS(CsA)-NP组显著强于CsA原药组及CS-NP组(均P<0.01)。结论 CS(CsA)-NP可缓慢释放药物,能有效抑制RCFs的增生,随时间延长,作用显著强于CsA原药,可作为缓释药物载体。

Objective To observe the character of cyclosporin a-loaded chitosan nanoparticles[CS （CsA）-NP],and study the inhibitive effect of CS（CsA）-NP in rabbit＇s conjunctival fibroblasts（RCFs） proliferation in vitro.Methods The rabbit＇s conjunctival fibroblasts were divided into four groups;CS （CsA）-NP,original CsA,blank chitosan nanoparticle（CS-NP） and the control group.Different concentrations of drugs were test.The incubation time of drug was 24～72 hours.The cells growth conditions were observed with light microscope and the inhibition of the drugs to the RCFs were determined with the method of methyl thiazolyl tetrazolium（MTT） colorimetric assay.Results CS（CsA）-NP,original CsA and CSNP inhibited RCFs proliferation in experimental groups in comparison with that in the control group in a dose-dependent and time- dependent manner.The inhibitive effect of CS（CsA） -NP was more effective than in original CsA and CS-NP（P〈0.01）.Conclusion CS（CsA）-NP can inhibit the proliferation of RCFs and can be be proposed as a potential controlled and targeted ophthalmic delivery system.