涎腺肿瘤组织中人乳头瘤病毒DNA的检测

DETECTION OF HUMAN PAPILLOMAVIRUS IN SALIVARY GLAND TUMORS

为探讨腺上皮起源的涎腺良、恶性肿瘤与人乳头瘤病毒（Ｈｕｍ ａｎ ｐａｐｉｌｌｏｍ ａｖｉｒｕｓ， ＨＰＶ）感染的关系，应用聚合酶链反应技术（ＰＣＲ），采用共有引物检测４４例涎腺肿瘤组织中的ＨＰＶ ＤＮＡ的存在状况。１０ 例正常涎腺组织作为对照。结果显示：肿瘤组织中ＨＰＶ ＤＮＡ 阳性检出率达７０．４５％ （３１／４４），其中恶性肿瘤 ＨＰＶＤＮＡ 阳性率为７７２７％ （３１／４４）；良性肿瘤ＨＰＶＤＮＡ阳性率为６３６３％ （１４／２２）；而正常对照组织中ＨＰＶ ＤＮＡ阳性率仅为１０％ （１／１０）。

To discover the relationship between human papillomavirus (HPV) and salivary gland tumor, the polymerase chain reaction (PCR) was used to detect HPV DNA in 44 cases of formalin fixed, paraffin embedded salivary gland tumor tissues and 10 cases of normal salivary gland tissues.The results showed that the total positive rate of HPV DNA was 70.45% (31/44);For benign tumor HPV DNA positive rate was 63.63%(14/22) and for malignant tumor was 77.27%(17/22).In contrast, HPV DNA positive rate was total to be 10%(1/10) in normal gland tissues (70.45% vs 10%,P<0.01).The results suggests that HPV plays a role in carcinogenesis of salivary gland tumor.