肝细胞癌组织和癌周组织中p53基因密码子249突变的比较研究

Comparison of codon 249 mutations of p53 gene in cancerous tissues with that in pericancerous tissues of HCCs

目的：本研究通过比较研究ＨＣＣ 癌组织和癌周组织中ｐ５３ 基因密码子２４９ 特征性突变来进一步阐明该基因在ＨＣＣ 发生机制中的意义。方法：收集９ 份癌组织中经单链构型多态性分析研究证实存在密码子２４９ 突变的ＨＣＣ 标本和１１ 例无此突变的ＨＣＣ 标本，采用等位基因特异性ＰＣＲ（ＡＳ－ ＰＣＲ） 方法比较研究癌组织和癌周组织中密码子２４９ 突变情况。结果：２０ 例ＨＣＣ 中，癌周组织中密码子２４９ 特征性突变检出率为７０ ％ 。９ 例ＳＳＣＰ 阳性者的癌周组织中的特征性突变检出率达１００ ％ 。１１ 例阴性患者的癌组织和癌周组织也能检出一定水平的突变。结论：ｐ５３基因密码子２４９ 突变在非癌肝组织中广泛存在。癌组织中密码了２４９ 特征性突变可能发生在肿瘤形成之前。

Objective:To analyze codon 249 mutation of p53 gene in cancerous tissue and pericancerous tissue of HCC comparatively,and study the significance of p53 gene in hepatocarcinogenesis.Methods:Codon 249 mutations were detected using allele specific PCR in cancerous and pericancerous tissues of 9 codon 249 mutation positive cases and 11 negative cases detected by single stranded conformational polymorphism (SSCP) analysis in former research.Results:The detective rate of codon 249 mutation in pericancerous tissues was 70%(14/20).That in 9 cases of SSCP positive HCCs was 100%(9/9).Codon 249 mutations were also detected in cancerous and pericancerous tissues of 11 SSCP negative HCCs.Conclusions:Codon 249 mutations of p53 gene were very popular in non neoplastic tissues.Those mutations in cancerous tissues might take place in the stage before the formation of tumor.