bcl 2基因高表达与乳腺癌生物学行为的相关性

HIGH EXPRESSION OF bcl 2 ONCOGENE IN BREAST CANCER AND ITS CORRELATION TO TUMOR BIOLOGICAL BEHAVIORS

目的 研究乳腺癌手术标本中ｂｃｌ２ 基因表达与乳腺癌生物学行为的关系。 方法 用免疫组化ＳＰ法检测乳腺癌手术标本中ｂｃｌ２ 基因产物的表达。同时检测雌二醇、ｐ５３ 基因及ｃｅｒｂＢ２ 基因的表达。 结果 １０５例乳腺癌原发灶中，ｂｃｌ２ 基因表达阳性率为４８．６ ％ ，ｐ５３ 基因、ｃｅｒｂＢ２ 基因及雌二醇表达率分别为３２．４ ％ 、５２．４ ％和５５．３ ％ ；ｂｃｌ２ 表达与乳腺癌患者年龄及区域淋巴结转移程度无关（ Ｐ＞ ０．０５） ；ｂｃｌ２ 基因表达与乳腺癌临床病理学分期进展及肿瘤大小呈负相关（Ｐ＜ ０．０５ ，Ｐ＜ ０．００５） ，而与肿瘤细胞分化程度正相关（ Ｐ＜ ０．００５） ；ｂｃｌ２ 基因表达与雌二醇密切正相关（ Ｐ＜ ０．００１） ，而与ｐ５３ 基因，ｃｅｒｂＢ２ 基因负相关（ Ｐ＜ ０．０５，Ｐ＜ ０．００５） 。ｂｃｌ２ 表达阳性的乳腺癌患者，术后三年、五年生存率显著高于ｂｃｌ２ 阴性者（ Ｐ＜ ０．００５） 。 结论 ｂｃｌ２ 基因参与乳腺癌生物学行为的调控，检测ｂｃｌ２ 基因表达状况，可以从另一个侧面了解乳腺癌的恶性程度，并有助于乳腺癌患者的预后评判。

To study the relationship of expression of bcl 2 gene product in human breast carcinomas to the biological behavior of breast cancer.METHODS immunohistochemistry with antibody recognising bcl 2 oestrogen and p53 c erb B2 encoded proteins were used.RESULTS bcl 2,p53,c erb B2 gene products and oestrogen were detected,respectively,in 48.6%,32.4%,52.4% and 55.3% of the 105 breast carcinomas examined.No relationship between the expresson of bcl gene to age and regional lymphnode metastasis( P >0.05) was found.However,a significant inverse correlation was seen between bcl 2 expression and P TNM stage ( P <0 005),tumor size ( P <0 05),histological grade ( P <0 005) and p53( P <0 05),c erb B2 gene ( P <0 05) expression. A significant positive correlation of bcl 2 with oestrogen and longer post operative survival ( P <0 005,by log rank test) was also found.CONCLUSIONSCONCLUSIONS Our resluts indicate that bcl 2 expression contributes to the biological behaviour of breast cancer.