晶状体上皮细胞PAI-1蛋白酶mRNA的表达

Expression of PAI-1 mRNA in bovine lens epithelial cells

目的 研究从转录水平确定晶状体上皮细胞是否能够合成纤溶酶原激活物抑制物 1(PAI 1)蛋白酶。方法 体外培养的牛晶状体上皮细胞 ,当细胞生长融合后 ,抽提RNA ;采用Nothernblot分析技术 ,对抽提得到的牛晶状体上皮细胞RNA用特异的cDNA探针进行检测。结果 在牛晶状体上皮细胞的基因转录产物中存在有PAI 1mRNA的表达 ,所表达的PAI 1mRNA碱基长度为 2 .3kb。结论 牛晶状体上皮细胞能够分泌PAI 1蛋白酶 ;

Objective To determine whether lens epithelial cells(LEC)might synthesize plasminogen activator inhibitor 1(PAI 1) enzyme at the transcription level. Methods Bovine LEC was cultured.Total RNA was isolated when LEC in primary culture became confluent.PAI 1 mRNA was identified by Nothern blot analysis using specific cDNA probes. Results There was expression of PAI 1 mRNA in the gene transcription products from bovine LEC.The size of mRNA observed was 2.3*!kb for PAI 1 transcripts. Conclusion Bovine LEC can secrete PAI 1 enzyme,which may play an important role in the occurrence of fibrin reaction after cataract extracapsular extraction.