应用肝素灌注预防后囊混浊的实验研究

An experimental study on the prevention of posterior capsular opacification by using heparinized irrigating solution

目的 研究肝素对兔眼白内障囊外摘出术 (ECCE)后后囊混浊的作用 ,以及肝素对角膜内皮、前房反应的影响。方法 建立白内障动物模型 ,在ECCE中应用不同浓度的肝素灌注液 ,术后应用裂隙灯显微镜、检眼镜及组织学方法观察角膜、前房及后囊膜的变化。结果 对照组与 5 0U/ml和 10 0U/ml肝素组角膜内皮水肿眼数及内皮细胞损失率无显著性差异 (P >0 .0 5 ) ,前房反应眼数及后囊混浊眼数对照组与两个肝素组有显著性差异 (P <0 .0 5 ) ,而两组肝素组之间无差异。结论 肝素可抑制后发障的形成 ,减少早期前房反应 。

Objective To investigate the effect of heparin on posterior capsular opacification,endothelial cells and anterior chamber reaction after extracapsular cataract extraction(ECCE)in rabbits. Methods The changes of corneal endothelial cells,anterior chamber and posterior capsule after ECCE were observed histologically by using slit lamp microscope and ophthalmoscope. Results Posterior capsular opacification developed 3 months after the operation.There was a significant difference in posterior capsular opacification and anterior chamber reaction between heparin treated group and control group( P <0.05),but there was no significant difference between endothelial cell edema and loss rate( P >0.05). Conclusion Heparin has a significant inhibitory effect on after cataract formation.It reduces anterior chamber reaction in early stage and not harmful to endothelial cells.It is readily available and is safe for preventing after cataract.