白藜芦醇对脑缺血再灌注后细胞凋亡及Caspase-3表达的影响

Effect of resveratrol on apoptosis and expression of Caspase-3 in rats with focal cerebral ischemia and reperfusion

目的研究白藜芦醇(Res)对大鼠局灶性脑缺血再灌注后神经细胞凋亡及Caspase-3蛋白表达的影响。方法 48只SD雄性大鼠随机分成4组,假手术组(S组)、缺血再灌注组(I/R组)、Res低剂量组(15 mg/kg,I/R﹢R1组)和Res高剂量组(30 mg/kg,I/R﹢R2组)。缺血2 h再灌注24 h,TTC法测量脑梗死体积,原位末端标记(TUNEI)法检测脑细胞凋亡水平,免疫组化法及Western blot检测Caspase-3蛋白表达,结果与I/R组相比,Res能明显抑制缺血区脑组织Caspase-3蛋白的表达(P<0.01),减少凋亡细胞数(P<0.01),缩小脑梗死体积(P<0.01)。结论 Res对大鼠局灶性脑缺血再灌注后神经细胞凋亡有抑制作用,其机制可能与Caspase-3表达下降有关。

AIM To study the effect of resveratrol on apoptosis and expression of Caspase-3 in rats following focal cerebral ischemia and reperfusion,and explore the possible mechanism of resveratrol protect on cerebral ischemia-reperfusion injury.METHODS Forty-eight male SD rats were randomly divided into four groups：sham operation group（S group）,ischemia/reperfusion group（I/R group）,low dose of resveratrol（15 mg / kg,I/R ﹢ R1 group） and high dose of resveratrol（30 mg / kg,I/R ﹢ R2 group）.In middle cerebral artery occlusion（for） 2 h and reperfusion for 24 h,2,3,5-triphenyltetrazolium chloride was used to detecte infarct volume.TUNEL was used to measure apoptosis.Immunohistochemistry and Western blot were used to analyse the protein expression of Caspase-3.RESULTS Compared with I/R group,resveratrol significantly decreased the protein expression of Caspase-3 in ischemia brain tissue（P 0.01）,reduced the number of apoptotic cells and the infarct volume（P 0.01）.CONCLUSION Resveratrol can inhibit neuronal apoptosis in rats following focal cerebral ischemia and reperfusion,the mechanism may be related to decreased expression of Caspase-3.