HPLC法测定玄麦甘桔颗粒中甘草酸和哈巴俄苷

Quantitative determination of glycyrrhitate and harpagoside in Xuanmai Ganjie Granules

目的建立玄麦甘桔颗粒(玄参、麦冬、桔梗、甘草)中甘草酸和哈巴俄苷的测定方法。方法采用HPLC法测定玄麦甘桔颗粒中甘草酸和哈巴俄苷。色谱柱:C18柱,流动相:乙腈-0.2 mol/L醋酸铵溶液-冰醋酸(30∶70∶1)(甘草酸)、甲醇-1%醋酸水溶液(50∶50)(哈巴俄苷);检测波长为252 nm(甘草酸)、278 nm(哈巴俄苷)。结果甘草酸在0.021～4.298μg的范围内,线性关系良好(r=0.999 8)。平均回收率为102.8%,RSD为0.90%。哈巴俄苷在0.025～0.841μg的范围内,线性关系良好(r=0.999 9)。平均回收率为98.2%,RSD为1.83%。结论甘草酸与哈巴俄苷的定量测定方法专属性强、重复性好。

AIM To establish quantitative determination methods of glycyrrhitate and harpagoside in Xuanmai ganjie Granules（Scrophulariae Radix,Ophiopogonis Radix,Platycodonis Radix,Glycyrrhizae Radix et Rhizoma）.METHODS HPLC with C18 chromatographic column was used in determination of glycyrrhitate and harpagoside.The mobilie phases were acetonitrile-0.2 mol/L ammonium acetate solution-glacial acetic acid（30 ∶ 70 ∶ 1）（glycyrrhitate）、methanol-1% acetic acid solution（50 ∶ 50）（harpagoside）;the detection wavelengths were 252 nm for glycyrrhizate and 278 nm for harpagoside.RESULTS The linear range of glycyrrhitate was 0.021-4.298 μg（r =0.999 8）,the average recovery was 102.8% with RSD of 0.90%.The linear range of harpagoside was 0.025-0.841 μg（r = 0.999 9）,the average recovery was 98.2% with RSD of 1.83%.CONCLUSION These quantitative determination methods are reproducible and specific.