硫化氢对MPP^+诱导PC12细胞氧化应激损伤的保护作用

Protective Effect of Hydrogen Sulfide on MPP^+-Induced Oxidative Stress Damage in PC12 Cells

目的探讨硫化氢对MPP+诱导PC12细胞氧化应激损伤的保护作用。方法以MPP+损伤PC12细胞作为帕金森病的细胞模型,甲氮甲唑蓝(MTT)法检测细胞存活率;丙酮酸二硝基苯腙比色法检测细胞上清液乳酸脱氢酶(LDH)漏出量;硫代巴比妥法检测细胞上清液中丙二醛(MDA)浓度;双氢罗丹明123染色FCM检测细胞内活性氧水平变化;应用硫化氢钠(sodium hydrosulfide,NaHS)作为H2S的供体。结果 200μmol/L和400μmol/L硫氢化钠呈浓度依赖性阻断MPP+引起PC12细胞存活率的降低;400μmol/L硫氢化钠能明显抑制400μmol/L MPP+诱导的PC12细胞LDH的漏出,以及抑制MDA和活性氧的产生。结论硫化氢对MPP+诱导PC12细胞的氧化应激损伤具有保护作用。

Objective To study the protective effect of hydrogen sulfide（H2S）on rat PC12 cells oxidative stress damage induced by 1-Methyl 4-phenylpyridiniumion（MPP＋）in vitro.Methods Using MPP＋ induced PC12 cells damage as the cell model of Parkinsons disease;The viability of PC12 cells was observed by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay;The transudation content of lactate dehydregenase（LDH）was measured by colorimetrically;The level of malondialde-hyde（MDA）in supernatant-conditioned media was monitored by thiobituric acid reaction（TBA）.The level of reactive oxygen species（ROS）in PC12 cells was tested by dihydrohodamine 123 stain FCM;Sodium hydrosulfide（NaHS）was used as H2S donor.Results NaHS（200 and 400 μmol/L）dose-dependently blocked the inhibition of cell viability induced by MPP＋;400 μmol NaHS significantly inhibited the loss of LDH,the overproduction of MDA and ROS in PC12.Conclusion H2S has the protective effect on PC12 cells oxidative stress damage induced by MPP＋.