慢性胃炎与胃癌组织中微血管密度和黏附分子表达的对比研究

Comparative study on the expression of microvessel density and adhesion molecules in the tissueof chronic gastritis and gastric cancer

目的 探讨慢性胃炎与胃癌组织中微血管密度、黏附分子的表达及对比意义.方法 选取56例慢性浅表性胃炎患者（慢性浅表性胃炎组）、55例胃癌无淋巴结转移患者（胃癌无淋巴结转移组）,57例胃癌有淋巴结转移患者（胃癌有淋巴结转移组）的病理组织蜡块,用免疫组织化学Envision法显示微血管密度CD34和细胞黏附分子Syndecan-1的表达情况,112例胃癌患者中高、中分化47例,低分化65例.结果 CD14表达以微血管密度计算：慢性浅表性胃炎组为（13 ± 10）个/高倍镜视野（HP）,胃癌无淋巴结转移组为（27 ± 11）个/HP;胃癌有淋巴结转移组为（28 ±10）个/HP.慢性浅表性胃炎组与胃癌无淋巴结转移组及胃癌有淋巴结转移组比较差异有统计学意义（t=7.044、7.908,P＜0.05）,胃癌无淋巴结转移组与胃癌有淋巴结转移组比较差异无统计学意义（t=0.506,P＞0.05.慢性浅表性胃炎组、胃癌无淋巴结转移组、胃癌有淋巴结转移组Syndecan-1阳性表达率分别为96.43%（54/56）、45.45%（25/55）、24.56%（14/57）,慢性浅表性胃炎组与胃癌无淋巴结转移组及胃癌有淋巴结转移组比较差异有统计学意义（X2=163.560、17.197,P＜0.01）.胃癌无淋巴结转移组与胃癌有淋巴结转移组比较差异有统计学意义（x2=5.340,P＜0.05）.结论 微血管密度增加与胃癌的发生有关;细胞黏附分子syndecan-1表达下调与胃癌的发生、发展有关,并可能进一步促进胃癌转移,检测微血管密度CD34及细胞黏附分子Syndecan-1的表达变化情况有助于胃癌的诊断及预后判断.

Objective To investigate the clinical significance of the expression of microvessel density and adhesion molecules in the tissue of chronic gastritis and gastric cancer. Methods Fifty-six patients with chronic superficial gastritis （chronic superficial gastritis group）, 55 cases of gastric cancer without lymph node metastasis （gastric cancer without lymph node metastasis group） and 57 patients with gastric cancer and lymph node metastasis （gastric cancer with lymph node metastasis group） were included in this study. Gastric pathology paraffin blocks was studied using immunohistochemical Envision method. Microvessel density CD34 and cell adhesion molecule Syndecan-1 expression was examined. In 112 gastric cancer patients, 47 patients were highly differentiated, and 65 patients were poorly differentiated. Results Microvessel density CD34 results：in chronic superficial gastritis group was （13 ± 10）/HP,in gastric cancer without lymph node metastasis group was （27 ± 11 VHP, in gastric cancer with lymph node metastasis group was （28 ± 10）/HP. There were significant differences between chronic superficial gastritis group and gastric cancer without lymph node metastasis group,and between chronic superficial gastritis group and gastric cancer with lymph node metastasis group （t = 7.044,7.908, P 〈0.05）, but there was no significant difference between gastric cancer without lymph node metastasis group and gastric cancer with lymph node metastasis group （t = 0.506,P 〉 0.05）. In chronic superficial gastritis group, gastric cancer without lymph node metastasis group and gastric cancer with lymph node metastasis group, Syndecan-1 positive expression ratewas 96.43%（54/56）,45.45%（25/55）,24.56%（14/57）,and there was significant difference between chronic superficial gastritis group and gastric cancer without lymph node metastasis group,and between chronic superficial gastritis group and gastric cancer with lymph node metastasis group （x2 = 163.560,17.197,P〈 0.01）. There was significant difference between gastric cancer without lymph node metastasis group and gastric cancer with lymph node metastasis group （x2 = 5.340, P 〈 0.05）. Conclusions Microvessel density is related with the occurrence of gastric cancer;adhesion molecule Syndecan-1 expression reduces the incidence of gastric cancer development, and might further promote the metastasis of gastric cancer. Detecting the expression changes of microvessel density CD34 and cell adhesion molecule Syndecan-1 facilitates the diagnosis and prognosis of gastric cancer.